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Differential detection and genetic relatedness of phytoplasmas in papaya

Gibb, KS, Schneider, BL and Padovan, A (1998). Differential detection and genetic relatedness of phytoplasmas in papaya. Plant Pathology,47(3):325-332.

Document type: Journal Article
Citation counts: Scopus Citation Count Cited 22 times in Scopus Article | Citations

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Title Differential detection and genetic relatedness of phytoplasmas in papaya
Author Gibb, KS
Schneider, BL
Padovan, A
Journal Name Plant Pathology
Publication Date 1998
Volume Number 47
Issue Number 3
ISSN 0032-0862   (check CDU catalogue open catalogue search in new window)
Scopus ID 2-s2.0-0031859562
Start Page 325
End Page 332
Total Pages 8
Place of Publication Oxford, UK
Publisher Blackwell Science
HERDC Category C1 - Journal Article (DEST)
Abstract The genetic relatedness of phytoplasmas associated with dieback (PDB), yellow crinkle (PYC) and mosaic (PM) diseases in papaya was studied by restriction fragment length polymorphism (RFLP) analysis of the 16S rRNA gene and 16S rRNA/23S rRNA spacer region (SR). RFLP and SR sequence comparisons indicated that PYC and PM phytoplasmas were identical and most closely related to members of the faba bean phyllody strain cluster. By comparison the PDB phytoplasma was most closely related to Phormium yellow leaf (PYL) phytoplasma from New Zealand and the Australian grapevine yellows (AGY) phytoplasma from Australia. These three phytoplasmas cluster with the stolbur and German grapevine yellows (VK) phytoplasmas within the aster yellows strain cluster. Primers based on the phytoplasma tuf gene, which amplify gene products from members of the AY strain cluster, also amplified a DNA product from the PDB phytoplasma but not from either the PYC or PM phytoplasmas. Primers deduced from the 16S rRNA/SR selectively amplified rDNA sequences from the PDB and AGY phytoplasmas but not from other members of the stolbur strain cluster. Similarly, primers designed from 16S rRNA/SR amplified rDNA from the PYC and PM phytoplasmas but not from the PDB phytoplasma. These primers may provide for more specific detection of these pathogens in epidemiological studies.
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