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Detection and identification of a phytoplasma from lucerne with Australian lucerne yellows disease

Pilkington, LJ, Gibb, KS, Gurr, GM, Fletcher, MJ, Nikandrow, A, Elliott, E, Van De Ven, R and Read, DMY (2003). Detection and identification of a phytoplasma from lucerne with Australian lucerne yellows disease. Plant Pathology,52(6):754-762.

Document type: Journal Article
Citation counts: Scopus Citation Count Cited 8 times in Scopus Article | Citations

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Title Detection and identification of a phytoplasma from lucerne with Australian lucerne yellows disease
Author Pilkington, LJ
Gibb, KS
Gurr, GM
Fletcher, MJ
Nikandrow, A
Elliott, E
Van De Ven, R
Read, DMY
Journal Name Plant Pathology
Publication Date 2003
Volume Number 52
Issue Number 6
ISSN 1365-3059   (check CDU catalogue open catalogue search in new window)
Scopus ID 2-s2.0-0345770363
Start Page 754
End Page 762
Total Pages 9
Place of Publication Oxford, England
Publisher Blackwell Publishing
Field of Research 0605 - Microbiology
0607 - Plant Biology
HERDC Category C1 - Journal Article (DEST)
Abstract Foliar and root symptoms are described for Australian lucerne yellows (ALuY), a disease common in Australian lucerne seed crops. A phytoplasma was detected in plants exhibiting symptoms, but not in symptomless lucerne plants. Oligonucleotide primers specific to the phytoplasma 16S-23S rRNA intergenic spacer region (SR) were used in polymerase chain reaction (PCR) assays on DNA extracted from lucerne plants with and without symptoms. Identical restriction fragment length polymorphism (RFLP) enzyme profiles were obtained for PCR products amplified from 10 yellows-affected lucerne samples. RFLP profiles obtained for four restriction enzymes were different from those of the tomato big bud (TBB) phytoplasma. ALuY phytoplasma PCR products were sequenced to determine phylogeny and were found to fall within the faba bean phyllody phytoplasma group, or phytoplasma group 16srII. Transmission electron microscopy revealed phytoplasmas in the phloem of yellows-affected plant samples, but not in symptomless plant samples. Fungal, bacterial and viral agents in the aetiology of Australian lucerne yellows were ruled out.
Keywords alfalfa
faba bean phyllody phytoplasma
medicago sativa
yellows disease
16s ribosomal-rna
rhodococcus fascians
pcr amplification
transient streak
leaf phytoplasma
aster yellows
differentiation
genes
classification
plant
DOI http://dx.doi.org/10.1111/j.1365-3059.2003.00934.x   (check subscription with CDU E-Gateway service for CDU Staff and Students  check subscription with CDU E-Gateway in new window)
 
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