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Contamination in HPLC quantified benzaldehyde is from polypropylene microtubes

Shemesh, T, Karschimkus, C and Rowley, KG (2005). Contamination in HPLC quantified benzaldehyde is from polypropylene microtubes. Journal of Chromatography B: analytical technologies in the biomedical and life sciences,826(1-2):277-278.

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Title Contamination in HPLC quantified benzaldehyde is from polypropylene microtubes
Author Shemesh, T
Karschimkus, C
Rowley, KG
Journal Name Journal of Chromatography B: analytical technologies in the biomedical and life sciences
Publication Date 2005
Volume Number 826
Issue Number 1-2
ISSN 1570-0232   (check CDU catalogue open catalogue search in new window)
Start Page 277
End Page 278
Place of Publication Netherlands
Publisher Elsevier
HERDC Category C1 - Journal Article (DEST)
Abstract Semicarbazide-sensitive amine oxidase (SSAO; EC 1.4.3.6) is a copper-containing enzyme predominantly expressed by vascular smooth muscle cells. SSAO deaminates primary amines to produce aldehydes and oxygen peroxides, and may thus play a role in vascular damage. SSAO activity can be quantified by assaying benzaldehyde production using fluorescent derivatisation and separation by HPLC. We performed the derivatisation step in polypropylene or borosilicate glass tubes over 45 min at 95 degrees C. High and obstructing background levels of benzaldehyde were found in one batch of polypropylene vials, as opposed to its alternatives. Treatment and handling of product shipment into the country did not account for introduction of contaminant into packaged vials nor did any reagent used in the assay. We conclude that the source of contamination was most likely due to variation in the commercial production process. Use of borosilicate vials for assays based on aldehyde production and derivatisation is recommended. (c) 2005 Elsevier B.V. All rights reserved.
Keywords semicarbazide-sensitive amine oxidase
hplc
benzaldehyde
sensitive amine oxidase
DOI http://dx.doi.org/10.1016/j.jchromb.2005.09.003   (check subscription with CDU E-Gateway service for CDU Staff and Students  check subscription with CDU E-Gateway in new window)
 
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