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Identification and characterization of Sarcoptes scabiei and Dermatophagoides pteronyssinus glutathione S-transferases: Implication as a potential major allergen in crusted scabies

Dougall, Annette, Holt, Deborah C., Fischer, Katia, Currie, Bart J., Kemp, David J. and Walton, Shelley F. (2005). Identification and characterization of Sarcoptes scabiei and Dermatophagoides pteronyssinus glutathione S-transferases: Implication as a potential major allergen in crusted scabies. American Journal of Tropical Medicine and Hygiene,73(5):977-984.

Document type: Journal Article
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IRMA ID 10239xPUB19
Title Identification and characterization of Sarcoptes scabiei and Dermatophagoides pteronyssinus glutathione S-transferases: Implication as a potential major allergen in crusted scabies
Author Dougall, Annette
Holt, Deborah C.
Fischer, Katia
Currie, Bart J.
Kemp, David J.
Walton, Shelley F.
Journal Name American Journal of Tropical Medicine and Hygiene
Publication Date 2005
Volume Number 73
Issue Number 5
ISSN 0002-9637   (check CDU catalogue open catalogue search in new window)
Start Page 977
End Page 984
Total Pages 8
Place of Publication US
Publisher American Society of Tropical Medicine and Hygiene
Field of Research 1117 - Public Health and Health Services
HERDC Category C1 - Journal Article (DEST)
Abstract The astigmatid mite Sarcoptes scabiei is the causative agent of scabies, a highly infectious parasitic disease of the skin. Although the mite causes marked hypersensitivity reactions, particularly in crusted (severe) scabies, little is known about the specific scabies mite molecules involved in such immunologic responses. We have identified six genes encoding scabies mite homologues of mu and delta-like glutathione S-transferases (GSTs) as well as novel house dust mite GSTs. A mu class S. scabiei GST was subcloned into a prokaryotic expression system. The purified recombinant protein rSsGST01 reacted strongly with IgE and IgG4 in sera from crusted scabies patients. This response was not observed with control antigens or with ordinary scabies and uninfested patient sera. In addition, the specific IgE response to rSsGST01 did not correlate with the total IgE level of the patient. These results suggest that GST may play a role in the pathophysiology associated with crusted scabies.
Description for Link Link to published version
URL http://www.ajtmh.org/cgi/reprint/73/5/977
 
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Created: Fri, 12 Sep 2008, 08:35:25 CST by Administrator