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Burkholderia pseudomallei strain type, based on pulsed-field gel electrophoresis, does not determine disease presentation in melioidosis

Cheng, Allen C., Day, N., Mayo, Mark J., Gal, Daniel and Currie, Bart J. (2005). Burkholderia pseudomallei strain type, based on pulsed-field gel electrophoresis, does not determine disease presentation in melioidosis. Microbes and Infection: a journal on infectious agents and host defenses,7(1):104-109.

Document type: Journal Article
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IRMA ID 10103xPUB22
Title Burkholderia pseudomallei strain type, based on pulsed-field gel electrophoresis, does not determine disease presentation in melioidosis
Author Cheng, Allen C.
Day, N.
Mayo, Mark J.
Gal, Daniel
Currie, Bart J.
Journal Name Microbes and Infection: a journal on infectious agents and host defenses
Publication Date 2005
Volume Number 7
Issue Number 1
ISSN 1286-4579   (check CDU catalogue open catalogue search in new window)
Start Page 104
End Page 109
Total Pages 6
Place of Publication France
Publisher Elsevier Masson
Field of Research 0605 - Microbiology
1107 - Immunology
1108 - Medical Microbiology
HERDC Category C1 - Journal Article (DEST)
Abstract Melioidosis, the infection due to Burkholderia pseudomallei, may present with a spectrum of severity and may affect any site in the body. Differential strain virulence and tropism suggested by previous studies would have implications for virulence and vaccine work. We explored clinical correlations using pulsed-field gel electrophoresis (PFGE) typing in a well-characterised clinical collection. Two methods of analysis were used based on band-based similarity values: first, a conventional cluster analysis formed by the unweighted paired group mean analysis, and second, an analysis of the distribution of the within-group and between-group Dice coefficient. Clinical isolates from 114 cases of melioidosis occurring in the Northern Territory, Australia were studied; 71 strain types were defined with a Simpson's index of 0.91. No correlation was found between strain type and disease severity or site of melioidosis on presentation, with no differences in similarity values found when comparing within and between-groups. In particular, isolates from patients with neurological melioidosis were not clustered. There was evidence of geographical localisation. This study suggests that the variation in strain type may not be as important as host and environmental factors in determining the pattern of disease.
Keywords melioidosis
burkholderia pseudomallei
australia
pulsed-field gel electrophoresis
bacterial typing techniques
virulence
DOI http://dx.doi.org/10.1016/j.micinf.2004.08.020   (check subscription with CDU E-Gateway service for CDU Staff and Students  check subscription with CDU E-Gateway in new window)
Additional Notes 3524 (Journal)
 
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