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Short report: Application of a polymerase chain reaction to detect Burkholderia pseudomallei in clinical specimens from patients with suspected melioidosis

Gal, Daniel, Mayo, Mark J., Spencer, E., Cheng, Allen C. and Currie, Bart J. (2005). Short report: Application of a polymerase chain reaction to detect Burkholderia pseudomallei in clinical specimens from patients with suspected melioidosis. American Journal of Tropical Medicine and Hygiene,73(6):1162-1164.

Document type: Journal Article
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Title Short report: Application of a polymerase chain reaction to detect Burkholderia pseudomallei in clinical specimens from patients with suspected melioidosis
Author Gal, Daniel
Mayo, Mark J.
Spencer, E.
Cheng, Allen C.
Currie, Bart J.
Journal Name American Journal of Tropical Medicine and Hygiene
Publication Date 2005
Volume Number 73
Issue Number 6
ISSN 0002-9637   (check CDU catalogue  open catalogue search in new window)
Start Page 1162
End Page 1164
Place of Publication US
Publisher American Society of Tropical Medicine & Hygiene
HERDC Category C1 - Journal Article (DEST)
Abstract The diagnostic potential of a Burkholderia pseudomallei type three secretion system (TTS1) polymerase chain reaction (PCR) was examined on clinical specimens from 27 patients with sepsis in the Northern Territory of Australia, a region endemic for melioidosis. The TTS1 PCR was conducted on DNA extracted from a range of clinical specimens (blood, sputum, urine, joint, pericardial and pleural fluid, and swabs from skin lesions, throat, nose, and rectum). The PCR sensitivity in culture-positive clinical specimens from the nine confirmed patients with melioidosis was 65% and the specificity was 100%, with no PCR-positive results in specimens from 1.8 patients without melioidosis. The PCR based on the B. pseudomallei TTS1 has the potential to substantially improve the timeliness of diagnosis of melioidosis.
Keywords iii secretion genes
septicemic melioidosis
pseudomonas-pseudomallei
pcr
hybridization
diagnosis
identification
tests
 
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