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As a bacterial culture medium, citrated sheep blood agar is a practical alternative to citrated human blood agar in laboratories of developing countries

Russell, F. M., Biribo, S. S. N., Selvaraj, G., Oppedisano, F., Warren, S., Seduadua, A., Mulholland, Edward K. and Carapetis, Jonathan R. (2006). As a bacterial culture medium, citrated sheep blood agar is a practical alternative to citrated human blood agar in laboratories of developing countries. Journal of Clinical Microbiology,44(9):3346-3351.

Document type: Journal Article
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IRMA ID 10666xPUB35
Title As a bacterial culture medium, citrated sheep blood agar is a practical alternative to citrated human blood agar in laboratories of developing countries
Author Russell, F. M.
Biribo, S. S. N.
Selvaraj, G.
Oppedisano, F.
Warren, S.
Seduadua, A.
Mulholland, Edward K.
Carapetis, Jonathan R.
Journal Name Journal of Clinical Microbiology
Publication Date 2006
Volume Number 44
Issue Number 9
ISSN 0095-1137   (check CDU catalogue  open catalogue search in new window)
Start Page 3346
End Page 3351
Total Pages 6
Place of Publication Washington, United States
Publisher American Society for Microbiology
Field of Research 1108 - Medical Microbiology
0707 - Veterinary Sciences
0605 - Microbiology
HERDC Category C1 - Journal Article (DEST)
Abstract Human blood agar (HuBA) is widely used in developing countries for the isolation of bacteria from clinical specimens. This study compared citrated sheep blood agar (CSBA) and HuBA with defibrinated horse blood agar and defibrinated sheep blood agar (DSBA) for the isolation and antibiotic susceptibility testing of reference and clinical strains of Streptococcus pneumoniae, Streptococcus pyogenes, and Staphylococcus aureus. Reference and clinical strains of all organisms were diluted in brain heart infusion and a clinical specimen of cerebrospinal fluid and cultured on all agars. Viable counts, colony morphology, and colony size were recorded. Susceptibility testing for S. pneumoniae and S. pyogenes was performed on defibrinated sheep blood Mueller-Hinton agar, citrated sheep blood Mueller-Hinton agar (CSB MHA), and human blood Mueller-Hinton agar plates. For all organisms, the colony numbers were similar on all agars. Substantially smaller colony sizes and absent or minimal hemolysis were noted on HuBA for all organisms. Antibiotic susceptibility results for S. pneumoniae were similar for the two sheep blood agars; however, larger zone sizes were displayed on HuBA, and quality control for the reference strain failed on HuBA. For S. pyogenes, larger zone sizes were demonstrated on HuBA and CSBA than on DSBA. Poor hemolysis made interpretation of the zone sizes difficult on HuBA. CSBA is an acceptable alternative for the isolation of these organisms. The characteristic morphology is not evident, and hemolysis is poor on HuBA; and so HuBA is not recommended for use for the isolation or the susceptibility testing of any of these organisms. CSB MHA may be suitable for use for the susceptibility testing of S. pneumoniae.
DOI http://dx.doi.org/10.1128/JCM.02631-05   (check subscription with CDU E-Gateway service for CDU Staff and Students  check subscription with CDU E-Gateway in new window)
Additional Notes Copyright by the American Society for Microbiology


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