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Nonrandom distribution of Burkholderia pseudomallei clones in relation to geographical location and virulence

Vesaratchavest, Mongkol, Tumapa, Sarinna, Day, Nicholas P. J., Wuthiekanun, Vanaporn, Chierakul, Wirongrong, Holden, Matthew T. G., White, Nicholas J., Currie, Bart J., Spratt, Brian G., Feil, Edward J. and Peacock, Sharon J. (2006). Nonrandom distribution of Burkholderia pseudomallei clones in relation to geographical location and virulence. Journal of Clinical Microbiology,44(7):2553-2557.

Document type: Journal Article
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IRMA ID 10139xPUB107
Title Nonrandom distribution of Burkholderia pseudomallei clones in relation to geographical location and virulence
Author Vesaratchavest, Mongkol
Tumapa, Sarinna
Day, Nicholas P. J.
Wuthiekanun, Vanaporn
Chierakul, Wirongrong
Holden, Matthew T. G.
White, Nicholas J.
Currie, Bart J.
Spratt, Brian G.
Feil, Edward J.
Peacock, Sharon J.
Journal Name Journal of Clinical Microbiology
Publication Date 2006
Volume Number 44
Issue Number 7
ISSN 0095-1137   (check CDU catalogue open catalogue search in new window)
Start Page 2553
End Page 2557
Total Pages 5
Place of Publication Washington, United States
Publisher American Society for Microbiology
Field of Research 0605 - Microbiology
0707 - Veterinary Sciences
1108 - Medical Microbiology
HERDC Category C1 - Journal Article (DEST)
Abstract Burkholderia pseudomallei is a soil-dwelling saprophyte and the causative agent of melioidosis, a life-threatening human infection. Most cases are reported from northeast Thailand and northern Australia. Using multilocus sequence typing (MLST), we have compared (i) soil and invasive isolates from northeast Thailand and (ii) invasive isolates from Thailand and Australia. A total of 266 Thai B. pseudomallei isolates were characterized (83 soil and 183 invasive). These corresponded to 123 sequence types (STs), the most abundant being ST70 (n = 21), ST167 (n = 15), ST54 (n = 12), and ST58 (n = 11). Two clusters of related STs (clonal complexes) were identified; the larger clonal complex (CC48) did not conform to a simple pattern of radial expansion from an assumed ancestor, while a second (CC70) corresponded to a simple radial expansion from ST70. Despite the large number of STs, overall nucleotide diversity was low. Of the Thai isolates, those isolated from patients with melioidosis were overrepresented in the 10 largest clones (P < 0.0001). There was a significant difference in the classification index between environmental and disease isolates (P < 0.001), confirming that genotypes were not distributed randomly between the two samples. MLST profiles for 158 isolates from Australia (mainly disease associated) contained a number of STs (96) similar to that seen with the Thai invasive isolates, but no ST was found in both populations. There were also differences in diversity and allele frequency distribution between the two populations. This analysis reveals strong genetic differentiation on the basis of geographical isolation and a significant differentiation on the basis of virulence potential.
Keywords burkholderia pseudomallei
DOI   (check subscription with CDU E-Gateway service for CDU Staff and Students  check subscription with CDU E-Gateway in new window)
Additional Notes Copyright of the American Society for Microbiology

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