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Clinical evaluation of a type III secretion system real-time PCR assay for diagnosing melioidosis

Meumann, Ella M., Novak, Ryan T., Gal, Daniel, Kaestli, Mirjam E., Mayo, Mark J., Hanson, Joshua P., Spencer, Emma, Glass, Mindy B., Gee, Jay E., Wilkins, Patricia P. and Currie, Bart J. (2006). Clinical evaluation of a type III secretion system real-time PCR assay for diagnosing melioidosis. Journal of Clinical Microbiology,44(8):3028-3030.

Document type: Journal Article
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IRMA ID 10582xPUB11
Title Clinical evaluation of a type III secretion system real-time PCR assay for diagnosing melioidosis
Author Meumann, Ella M.
Novak, Ryan T.
Gal, Daniel
Kaestli, Mirjam E.
Mayo, Mark J.
Hanson, Joshua P.
Spencer, Emma
Glass, Mindy B.
Gee, Jay E.
Wilkins, Patricia P.
Currie, Bart J.
Journal Name Journal of Clinical Microbiology
Publication Date 2006
Volume Number 44
Issue Number 8
ISSN 0095-1137   (check CDU catalogue  open catalogue search in new window)
Start Page 3028
End Page 3030
Total Pages 3
Place of Publication Washington, United States
Publisher American Society for Microbiology
Field of Research 1108 - Medical Microbiology
0605 - Microbiology
0707 - Veterinary Sciences
HERDC Category C1 - Journal Article (DEST)
Abstract A Burkholderia pseudomallei type III secretion system real-time PCR assay was evaluated on clinical specimens in a region where melioidosis is endemic. The PCR was positive in 30/33 (91%) patients with culture-confirmed melioidosis. All six patients with melioidosis septic shock were blood PCR positive, suggesting potential for rapid diagnosis and commencement of appropriate therapy.
Keywords Burkholderia pseudomallei
melioidosis
northern Australia
Southeast Asia
DOI http://dx.doi.org/10.1128/JCM.00913-06   (check subscription with CDU E-Gateway service for CDU Staff and Students  check subscription with CDU E-Gateway in new window)
Additional Notes Copyright by the Society for Microbiology


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