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B-cell antigen D8/17 is a marker of rheumatic fever susceptibility in Aboriginal Australians and can be tested in remote settings

Harrington, Zinta, Visvanathan, Kumar, Skinner, Narelle A., Curtis, Nigel, Currie, Bart J. and Carapetis, Jonathan R. (2006). B-cell antigen D8/17 is a marker of rheumatic fever susceptibility in Aboriginal Australians and can be tested in remote settings. Medical Journal of Australia,184(10):507-510.

Document type: Journal Article
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IRMA ID 10139xPUB127
Title B-cell antigen D8/17 is a marker of rheumatic fever susceptibility in Aboriginal Australians and can be tested in remote settings
Author Harrington, Zinta
Visvanathan, Kumar
Skinner, Narelle A.
Curtis, Nigel
Currie, Bart J.
Carapetis, Jonathan R.
Journal Name Medical Journal of Australia
Publication Date 2006
Volume Number 184
Issue Number 10
ISSN 0025-729X   (check CDU catalogue open catalogue search in new window)
Start Page 507
End Page 510
Total Pages 4
Place of Publication Australia
Publisher Australasian Medical Publishing Company Pty. Ltd.
Field of Research 1117 - Public Health and Health Services
HERDC Category C1 - Journal Article (DEST)
Abstract Objective:
To test the B-cell antigen D8/17 as a marker of past rheumatic fever (RF) in a predominantly Aboriginal Australian population, and to evaluate technical modifications to allow its use in remote settings.

Design and setting:
Cross-sectional survey in a remote Aboriginal community, a regional tertiary referral hospital and a tertiary paediatric centre in Melbourne.

Participants:
106 people, including three with acute RF, 38 with a history of past RF, 20 relatives of these people, and 45 healthy controls.

Main outcome measure:
D8/17 expression in B cells.

Results:
Blood was collected from each participant and the expression of D8/17 and CD19 in each sample was analysed by flow cytometry. The mean proportion of D8/17-positive B cells was 39.3% (SD, 11.8) in patients with previous RF, 22.5% (SD, 5.2) in first-degree relatives, 11.6% (SD, 7.2) in controls, and 83.7% (SD, 10.1) in patients with acute RF (analysis of variance test between means, P = 0.001). A cut-off of 22.1% of D8/17-positive B cells to indicate past RF yielded the highest percentage of correct results (95.4%). Delayed staining of whole blood (mean, 0.55 days; SD, 0.2) gave equivalent results to immediate staining, but the D8/17 assay on peripheral blood mononuclear cells was unreliable.

Conclusions:
The B-cell antigen D8/17 accurately identifies Australians with a past history of RF, and the assay is feasible in remote settings with access to facilities capable of performing D8/17 staining within half a day of sample collection.
Description for Link Link to published version
URL https://www.mja.com.au/journal/2006/184/10/b-cell-antigen-d817-marker-rheumatic-fever-susceptibility-aboriginal-australians


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