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Use of a variable amplicon typing scheme reveals considerable variation in the accessory genomes of isolates of Burkholderia pseudomallei

Duangsonk, Kwanjit, Gal, Daniel, Mayo, Mark, Hart, C. Anthony, Currie, Bart J. and Winstanley, Craig (2006). Use of a variable amplicon typing scheme reveals considerable variation in the accessory genomes of isolates of Burkholderia pseudomallei. Journal of Clinical Microbiology,44(4):1323-1334.

Document type: Journal Article
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IRMA ID 10103xPUB26
Title Use of a variable amplicon typing scheme reveals considerable variation in the accessory genomes of isolates of Burkholderia pseudomallei
Author Duangsonk, Kwanjit
Gal, Daniel
Mayo, Mark
Hart, C. Anthony
Currie, Bart J.
Winstanley, Craig
Journal Name Journal of Clinical Microbiology
Publication Date 2006
Volume Number 44
Issue Number 4
ISSN 0095-1137   (check CDU catalogue  open catalogue search in new window)
Start Page 1323
End Page 1334
Total Pages 12
Place of Publication Washington, United States
Publisher American Society for Microbiology
Field of Research 1108 - Medical Microbiology
0605 - Microbiology
0707 - Veterinary Sciences
HERDC Category C1 - Journal Article (DEST)
Abstract Melioidosis, a disease caused by the bacterium Burkholderia pseudomallei, is endemic in southeast Asia and northern Australia. We used suppression subtractive hybridization (SSH) to identify sequences that varied between two B. pseudomallei isolates from Australia and determined the distribution of 45 SSH-derived sequences among a panel of B. pseudomallei and B. thailandensis isolates. Sequences exhibiting variable prevalence were included in a variable amplicon typing (VAT) scheme designed to score the presence or absence of 14 PCR amplicons. VAT analysis was carried out with 48 isolates from Thailand, which were typed by multilocus sequence typing (MLST), and 44 isolates from Australia of known MLST type. The VAT scheme could be used to divide the 48 isolates from Thailand into 23 VAT types and the 44 isolates from Australia into 36 VAT types. Some of the sequences included in the VAT scheme were more commonly PCR positive among isolates from Australia than among isolates from Thailand, and vice versa. No isolate from Australia was PCR positive for genomic island 11 or a putative transposase sequence, whereas four SSH-derived sequences were far more prevalent among the Australian isolates. Analysis based on the VAT scheme indicated that the isolates clustered into groups, some of which were mainly or exclusively from one geographical origin. One cluster included Australian isolates that were mostly associated with severe disease, including rare neurological melioidosis, suggesting that the content of the accessory genome may play an important role in determining the clinical manifestation of the disease.
Keywords Burkholderia pseudomallei
melioidosis
northern Australia
Southeast Asia
DOI http://dx.doi.org/10.1128/JCM.44.4.1323-1334.2006   (check subscription with CDU E-Gateway service for CDU Staff and Students  check subscription with CDU E-Gateway in new window)
Additional Notes Copyright by the American Society for Microbiology


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