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An alternative method for sputum storage and transport for Mycobacterium tuberculosis drug resistance surveys

Lumb, R., Ardian, M., Waramori, G., Syahrial, H., Tjitra, E., Maguire, Graeme and Anstey, Nicholas M. (2006). An alternative method for sputum storage and transport for Mycobacterium tuberculosis drug resistance surveys. International Journal of Tuberculosis and Lung Disease,10(2):172-177.

Document type: Journal Article
Citation counts: TR Web of Science Citation Count  Cited 15 times in Thomson Reuters Web of Science Article | Citations
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ISI LOC 000235232600010
IRMA ID 10002xPUB51
Title An alternative method for sputum storage and transport for Mycobacterium tuberculosis drug resistance surveys
Author Lumb, R.
Ardian, M.
Waramori, G.
Syahrial, H.
Tjitra, E.
Maguire, Graeme
Anstey, Nicholas M.
Journal Name International Journal of Tuberculosis and Lung Disease
Publication Date 2006
Volume Number 10
Issue Number 2
ISSN 1027-3719   (check CDU catalogue open catalogue search in new window)
Start Page 172
End Page 177
Total Pages 6
Place of Publication France
Publisher International Union against Tuberculosis and Lung Disease
Field of Research 1102 - Cardiovascular Medicine and Haematology
HERDC Category C1 - Journal Article (DEST)
Abstract SETTING: A district level tuberculosis (TB) programme in Indonesia. OBJECTIVE: To evaluate whether a single sputum specimen could be stored by refrigeration for an extended period of time, then transported to a reference laboratory and successfully cultured for Mycobacterium tuberculosis. METHODS: Single sputum specimens were collected from newly diagnosed smear-positive pulmonary TB patients, refrigerated at the study site without addition of 1% cetylpyridinium chloride, hatched and sent to the reference laboratory, where they were decontaminated and inoculated into BACTEC MGIT 960 liquid media. RESULTS: One hundred and seven patients were enrolled. The median specimen storage time was 12 days (range 1-38) and median transportation time was 4 days (2-12). The median time from specimen collection until processing was 18 days (4-42). Only 4 (3.7%) specimens failed to grow Mycobacterium species and M. tuberculosis was isolated from 101 (94.4%) specimens. Six specimens with breakthrough contamination successfully grew M. tuberculosis after a second decontamination procedure. CONCLUSIONS: Single sputum specimens collected at a remote setting, refrigerated for relatively long periods without preservatives and transported without refrigeration to a reference laboratory can yield a high positive culture rate. These findings offer potential logistic simplification and cost savings for drug resistance surveys in low-resource countries.
Keywords tuberculosis
drug resistance
survey
sputum
specimens
cetyl-pyridinium chloride
bactec mgit 960
clinical specimens
recovery
culture
multicenter
 
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