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Short report: Lipopolysaccharide heterogeneity among Burkholderia pseudomallei from different geographic and clinical origins

Anuntagool, N., Wuthiekanun, Vanaporn, White, Nicholas J., Currie, Bart J., Sermswan, R. W., Wongratanacheewin, S., Taweechaisupapong, S., Chaiyaroj, S. C. and Strisinha, S. (2006). Short report: Lipopolysaccharide heterogeneity among Burkholderia pseudomallei from different geographic and clinical origins. American Journal of Tropical Medicine and Hygiene,74(3):348-352.

Document type: Journal Article
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Title Short report: Lipopolysaccharide heterogeneity among Burkholderia pseudomallei from different geographic and clinical origins
Author Anuntagool, N.
Wuthiekanun, Vanaporn
White, Nicholas J.
Currie, Bart J.
Sermswan, R. W.
Wongratanacheewin, S.
Taweechaisupapong, S.
Chaiyaroj, S. C.
Strisinha, S.
Journal Name American Journal of Tropical Medicine and Hygiene
Publication Date 2006
Volume Number 74
Issue Number 3
ISSN 0002-9637   (check CDU catalogue  open catalogue search in new window)
Start Page 348
End Page 352
Place of Publication US
Publisher The American Society of Tropical Medicine and Hygiene
HERDC Category C1 - Journal Article (DEST)
Abstract Heterogeneous patterns were obtained for lipopolysaccharide (LPS) from 1,327 Burkholderia pseudomallei isolates by sodium dodecyl sulfate-polyacryl amide gel electrophoresis, silver staining, and immunoblot analysis. Two LPS serotypes (A and B) possessing different ladder profiles and a rough LPS without ladder appearances were identified. All three LPS types were antigenically distinct by immunoblotting. The predominant type A (97%) produced the lowest amount of biofilm. The two less common types (smooth type B and rough type) were found more in clinical than environmental isolates and more in Australian isolates than Thai isolates. These isolates were more often associated with relapse than with primary infection.
Keywords biofilm formation
melioidosis
identification
diagnosis
virulence
antigens
thailand
 
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Created: Fri, 12 Sep 2008, 08:35:25 CST by Administrator