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BurkDiff: A Real-Time PCR Allelic Discrimination Assay for Burkholderia Pseudomallei and B. mallei

Bowers, Jolene R., Engelthaler, David M., Ginther, Jennifer L., Pearson, Talima, Peacock, Sharon J., Tuanyok, Apichai, Wagner, David M., Currie, Bart J. and Keim, Paul S. (2010). BurkDiff: A Real-Time PCR Allelic Discrimination Assay for Burkholderia Pseudomallei and B. mallei. PLoS One,5(11):e15413.

Document type: Journal Article
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Title BurkDiff: A Real-Time PCR Allelic Discrimination Assay for Burkholderia Pseudomallei and B. mallei
Author Bowers, Jolene R.
Engelthaler, David M.
Ginther, Jennifer L.
Pearson, Talima
Peacock, Sharon J.
Tuanyok, Apichai
Wagner, David M.
Currie, Bart J.
Keim, Paul S.
Journal Name PLoS One
Publication Date 2010
Volume Number 5
Issue Number 11
ISSN 19326203   (check CDU catalogue open catalogue search in new window)
Start Page e15413
Total Pages 7
Place of Publication United States
Publisher Public Library of Science
Abstract A real-time PCR assay, BurkDiff, was designed to target a unique conserved region in the B. pseudomallei and B. mallei genomes containing a SNP that differentiates the two species. Sensitivity and specificity were assessed by screening BurkDiff across 469 isolates of B. pseudomallei, 49 isolates of B. mallei, and 390 isolates of clinically relevant non-target species. Concordance of results with traditional speciation methods and no cross-reactivity to non-target species show BurkDiff is a robust, highly validated assay for the detection and differentiation of B. pseudomallei and B. mallei.
Keywords BurkDiff
B. pseudomallei
B. mallei
PCR assay
DOI http://dx.doi.org/10.1371/journal.pone.0015413   (check subscription with CDU E-Gateway service for CDU Staff and Students  check subscription with CDU E-Gateway in new window)


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Created: Fri, 05 Oct 2012, 11:40:59 CST by Teresa Haendel