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Increased transcription of Glutathione S-transferases in acaricide exposed scabies mites

Mounsey, Kate E., Pasay, Cielo J., Arlian, Larry G., Morgan, Marjorie S., Holt, Deborah C., Currie, Bart J., Walton, Shelley F. and McCarthy, James S. (2010). Increased transcription of Glutathione S-transferases in acaricide exposed scabies mites. Parasites & Vectors,3:43-51.

Document type: Journal Article
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IRMA ID 81704288xPUB42
Title Increased transcription of Glutathione S-transferases in acaricide exposed scabies mites
Author Mounsey, Kate E.
Pasay, Cielo J.
Arlian, Larry G.
Morgan, Marjorie S.
Holt, Deborah C.
Currie, Bart J.
Walton, Shelley F.
McCarthy, James S.
Journal Name Parasites & Vectors
Publication Date 2010
Volume Number 3
ISSN 1756-3305   (check CDU catalogue  open catalogue search in new window)
Start Page 43
End Page 51
Total Pages 9
Place of Publication United Kindom
Publisher BioMed Central Ltd.
HERDC Category C1 - Journal Article (DIISR)
Abstract Background: Recent evidence suggests that Sarcoptes scabiei var. hominis mites collected from scabies endemic communities in northern Australia show increasing tolerance to 5% permethrin and oral ivermectin. Previous findings have implicated detoxification pathways in developing resistance to these acaricides. We investigated the contribution of Glutathione S-transferase (GST) enzymes to permethrin and ivermectin tolerance in scabies mites using biochemical and molecular approaches.

Results: Increased in vitro survival following permethrin exposure was observed in S. scabiei var. hominis compared to acaricide naïve mites (p < 0.0001). The addition of the GST inhibitor diethyl maleate restored in vitro permethrin susceptibility, confirming GST involvement in permethrin detoxification. Assay of GST enzymatic activity in mites
demonstrated that S. scabiei var. hominis mites showed a two-fold increase in activity compared to naïve mites (p < 0.0001). Increased transcription of three different GST molecules was observed in permethrin resistant S. scabiei var. canis- mu 1 (p < 0.0001), delta 1 (p < 0.001), and delta 3 (p < 0.0001). mRNA levels of GST mu 1, delta 3 and Pglycoprotein also significantly increased in S. scabiei var. hominis mites collected from a recurrent crusted scabies patient over the course of ivermectin treatment.

Conclusions: These findings provide further support for the hypothesis that increased drug metabolism and efflux mediate permethrin and ivermectin resistance in scabies mites and highlight the threat of emerging acaricide resistance to the treatment of scabies worldwide. This is one of the first attempts to define specific genes involved in GST mediated acaricide resistance at the transcriptional level, and the first application of such studies to S. scabiei, a historically challenging ectoparasite.
Keywords Glutathione S-transferase (GST) enzymes
Sarcoptes scabiei var. hominis mites
increased transcription
DOI http://dx.doi.org/10.1186/1756-3305-3-43   (check subscription with CDU E-Gateway service for CDU Staff and Students  check subscription with CDU E-Gateway in new window)


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Created: Wed, 10 Oct 2012, 17:16:59 CST by Teresa Haendel