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Genomic islands from five strains of Burkholderia pseudomallei

Tuanyok, Apichai, Leadem, Benjamin R., Auerbach, Raymond K., Beckstrom-Sternberg, Stephen M., Beckstrom-Sternberg, James S., Mayo, Mark J., Wuthiekanun, Vanaporn, Brettin, Thomas S., Nierman, William C., Peacock, Sharon J., Currie, Bart J., Wagner, David M. and Keim, Paul (2008). Genomic islands from five strains of Burkholderia pseudomallei. BMC Genomics,9:566-579.

Document type: Journal Article
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IRMA ID 75039815xPUB103
Title Genomic islands from five strains of Burkholderia pseudomallei
Author Tuanyok, Apichai
Leadem, Benjamin R.
Auerbach, Raymond K.
Beckstrom-Sternberg, Stephen M.
Beckstrom-Sternberg, James S.
Mayo, Mark J.
Wuthiekanun, Vanaporn
Brettin, Thomas S.
Nierman, William C.
Peacock, Sharon J.
Currie, Bart J.
Wagner, David M.
Keim, Paul
Journal Name BMC Genomics
Publication Date 2008
Volume Number 9
ISSN 14712164   (check CDU catalogue open catalogue search in new window)
Start Page 566
End Page 579
Total Pages 14
Place of Publication London, U.K.
Publisher BioMed Central Ltd.
HERDC Category C1 - Journal Article (DEST)
Abstract Background: Burkholderia pseudomallei is the etiologic agent of melioidosis, a significant cause of morbidity and mortality where this infection is endemic. Genomic differences among strains of B. pseudomallei are predicted to be one of the major causes of the diverse clinical manifestations observed among patients with melioidosis. The purpose of this study was to examine the role of genomic islands (GIs) as sources of genomic diversity in this species.

Results: We found that genomic islands (GIs) vary greatly among B. pseudomallei strains. We identified 71 distinct GIs from the genome sequences of five reference strains of B. pseudomallei: K96243, 1710b, 1106a, MSHR668, and MSHR305. The genomic positions of these GIs are not random, as many of them are associated with tRNA gene loci. In particular, the 3' end sequences of tRNA genes are predicted to be involved in the integration of GIs. We propose the term "tRNAmediated site-specific recombination" (tRNA-SSR) for this mechanism. In addition, we provide a GI nomenclature that is based upon integration hotspots identified here or previously described.

Conclusion: Our data suggest that acquisition of GIs is one of the major sources of genomic diversity within B. pseudomallei and the molecular mechanisms that facilitate horizontally-acquired GIs are common across multiple strains of B. pseudomallei. The differential presence of the 71 GIs across multiple strains demonstrates the importance of these mobile elements for shaping the genetic composition of individual strains and populations within this bacterial species.
Keywords Burkholderia pseudomallei
etiologic agent of melioidosis
genomic islands (GIs)
DOI   (check subscription with CDU E-Gateway service for CDU Staff and Students  check subscription with CDU E-Gateway in new window)

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