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Incorrect identification of recent Asian strains of Coxsackievirus A16 as human enterovirus 71: Improved primers for the specific detection of human enterovirus 71 by RT PCR

Perera, David, Podin, Yuwana, Akin, Winnie, Tan, Cheng-Siang and Cardosa, Mary J. (2004). Incorrect identification of recent Asian strains of Coxsackievirus A16 as human enterovirus 71: Improved primers for the specific detection of human enterovirus 71 by RT PCR. BMC Infectious Diseases,4(1):11-21.

Document type: Journal Article
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Title Incorrect identification of recent Asian strains of Coxsackievirus A16 as human enterovirus 71: Improved primers for the specific detection of human enterovirus 71 by RT PCR
Author Perera, David
Podin, Yuwana
Akin, Winnie
Tan, Cheng-Siang
Cardosa, Mary J.
Journal Name BMC Infectious Diseases
Publication Date 2004
Volume Number 4
Issue Number 1
ISSN 1471-2334   (check CDU catalogue  open catalogue search in new window)
Start Page 11
End Page 21
Total Pages 11
Place of Publication United Kingdom
Publisher BioMed Central Ltd.
Abstract Background: Human enterovirus 71 has emerged as an important pathogen in the Asia Pacific region and it is important to be able to make a rapid and specific diagnosis for outbreak control. Recent Asian strains of Coxsackievirus A16 have changes in the VP1 gene which causes mispriming of widely used primers for human enterovirus 71 specific identification.

Methods: Local strains of Coxsackievirus A16 were sequenced in the VP4 and VP1 genes and using sequence alignment tools, an improved set of primers were designed for specific identification of human enterovirus 71. These primers were evaluated against virus isolates as well as primary clinical specimens.

Results: A total of 218 virus strains were tested. All 39 human enterovirus 71 isolates were positive and none of the 38 Coxsackievirus A16, 127 other enteroviruses and 14 prototype flaviviruses and adenoviruses were positive when tested with the new primers. When aliquots of primary specimens known to have yielded human enterovirus 71 were retrospectively tested, we found that within 2 months of collection of the specimens, greater than 90% were positive but that the success rate diminished rapidly to 18% after 2 years storage.

Conclusions: Our new primers will be useful in rapid diagnosis of human enterovirus 71 infection, and can also be used as a screening tool in surveillance programmes for early warning of human enterovirus 71 transmission.
Keywords Human enterovirus 71
Coxsackievirus A16
RT PCR
VP1 gene
improved primers
DOI http://dx.doi.org/10.1186/1471-2334-4-11   (check subscription with CDU E-Gateway service for CDU Staff and Students  check subscription with CDU E-Gateway in new window)


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