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Accuracy of enzyme-linked immunosorbent assay using crude and purified antigens for serodiagnosis of melioidosis

Chantratita, Narisara, Wuthiekanun, Vanaporn, Thanwisai, Aunchalee, Limmathurotsakul, Direk, Cheng, Allen C., Chierakul, Wirongrong, Day, Nicholas P. J. and Peacock, Sharon J. (2007). Accuracy of enzyme-linked immunosorbent assay using crude and purified antigens for serodiagnosis of melioidosis. Clinical and Vaccine Immunology,14(1):110-113.

Document type: Journal Article
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Title Accuracy of enzyme-linked immunosorbent assay using crude and purified antigens for serodiagnosis of melioidosis
Author Chantratita, Narisara
Wuthiekanun, Vanaporn
Thanwisai, Aunchalee
Limmathurotsakul, Direk
Cheng, Allen C.
Chierakul, Wirongrong
Day, Nicholas P. J.
Peacock, Sharon J.
Journal Name Clinical and Vaccine Immunology
Publication Date 2007
Volume Number 14
Issue Number 1
ISSN 1556-679X   (check CDU catalogue  open catalogue search in new window)
Start Page 110
End Page 113
Total Pages 4
Place of Publication Washington, United States
Publisher American Society for Microbiology
HERDC Category C1 - Journal Article (DEST)
Abstract Five enzyme-linked immunosorbent assays developed to detect antibodies to different Burkholderia Pseudomallei antigen preparations were evaluated as diagnostic tests for melioidosis in northeast Thailand. The highest diagnostic indices were observed for an affinity-purified antigen (sensitivity, 82%; specificity, 72%) and crude B. pseudomallei antigen (sensitivity, 81%; specificity, 70%), an improvement over the indirect hemagglutination assay (sensitivity, 73%; specificity, 64%).
Keywords Burkholderia pseudomallai
northeast Thailand
indirect hemagglutination
PSEUDOMONAS-PSEUDOMALLEI
serological diagnosis
area
lipopolysaccharide
polysaccaride
antibodies
virulence
DOI http://dx.doi.org/10.1128/CVI.00289-06   (check subscription with CDU E-Gateway service for CDU Staff and Students  check subscription with CDU E-Gateway in new window)


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