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An in vivo examination of the stability of venom from the Australian box jellyfish Chironex fleckeri

Winter, KL, Isbister, Gk, Seymour, JE and Hodgson, WC (2007). An in vivo examination of the stability of venom from the Australian box jellyfish Chironex fleckeri. Toxicon,49(6):804-809.

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Title An in vivo examination of the stability of venom from the Australian box jellyfish Chironex fleckeri
Author Winter, KL
Isbister, Gk
Seymour, JE
Hodgson, WC
Journal Name Toxicon
Publication Date 2007
Volume Number 49
Issue Number 6
ISSN 0041-0101   (check CDU catalogue open catalogue search in new window)
Start Page 804
End Page 809
Total Pages 6
Place of Publication UK
Publisher Pergamon
Field of Research 1115 - Pharmacology and Pharmaceutical Sciences
HERDC Category C1 - Journal Article (DEST)
Abstract We have previously characterised the pharmacological activity of a number of jellyfish venoms with a particular emphasis on the profound cardiovascular effects. It has been suggested that jellyfish venoms are difficult to work with and are sensitive to pH, temperature and chemical changes. The current study aimed to examine the working parameters of the venom of the Australian box jellyfish Chironex fleckeri to enable fractionation and isolation of the toxins with cardiovascular activity. C. fleckeri venom was made up fresh each day and subjected to a number of different environments (i.e. a pH range of 5–9 and a temperature range of 4–30 °C). In addition, the effect of freeze drying and reconstituting the venom was investigated. Venom (50 μg/kg, i.v.) produced a transient hypertensive response followed by cardiovascular collapse in anaesthetised rats. This biphasic response was not significantly effected by preparation of the venom at a pH of 5, 7 or 9. Similarly, venom (50 μg/kg, i.v.) did not display a loss of activity when exposed to temperatures of 4, 20 or 30 °C for 1.5 h. However, the cardiovascular activity was abolished by boiling the venom. Freeze drying, and then reconstituting, the venom did not significantly affect its cardiovascular activity. However, repeated freeze drying and reconstituting of extracted venom resulted in a significantly loss of activity. This study provides a more detailed knowledge of the parameters in which C. fleckeri venom can be used and, while supporting some previous studies, contradicts some of the perceived problems of working with the venom.
Keywords Jellyfish
Venom
Chironex fleckeri
Stability
Cardiovascular
DOI http://dx.doi.org/10.1016/j.toxicon.2006.11.031   (check subscription with CDU E-Gateway service for CDU Staff and Students  check subscription with CDU E-Gateway in new window)
 
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