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High throughput mRNA profiling characterizes the expression of inflammatory molecules in sepsis caused by Burkholderia pseudomallei

Weirsinga, W. Joost, Dessing, Mark C., Kager, Piet A., Cheng, Allen C., Limmaturotsakl, Direk, Day, Nicholas P. J., Dondorp, Arjen M., Van Der Poll, Tom and Peacock, Sharon J. (2007). High throughput mRNA profiling characterizes the expression of inflammatory molecules in sepsis caused by Burkholderia pseudomallei. Infection and Immunity,75(6):3074-3079.

Document type: Journal Article
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Title High throughput mRNA profiling characterizes the expression of inflammatory molecules in sepsis caused by Burkholderia pseudomallei
Author Weirsinga, W. Joost
Dessing, Mark C.
Kager, Piet A.
Cheng, Allen C.
Limmaturotsakl, Direk
Day, Nicholas P. J.
Dondorp, Arjen M.
Van Der Poll, Tom
Peacock, Sharon J.
Journal Name Infection and Immunity
Publication Date 2007
Volume Number 75
Issue Number 6
ISSN 0019-9567   (check CDU catalogue open catalogue search in new window)
Start Page 3074
End Page 3079
Total Pages 6
Place of Publication Washington, United States
Publisher American Society for Microbiology
HERDC Category C1 - Journal Article (DEST)
Abstract Sepsis is characterized by an uncontrolled inflammatory response to invading microorganisms. We describe the inflammatory mRNA profiles in whole-blood leukocytes, monocytes, and granulocytes using a multigene system for 35 inflammatory markers that included pro- and anti-inflammatory cytokines, chemokines, and signal transduction molecules in a case-control study with 34 patients with sepsis caused by the gram-negative bacterium Burkholderia pseudomallei (the pathogen causing melioidosis) and 32 healthy volunteers. Relative to healthy controls, patients with sepsis showed increased transcription of a whole array of inflammatory genes in peripheral blood leukocytes, granulocytes, and monocytes. Specific monocyte and granulocyte mRNA profiles were identified. Strong correlations were found between inflammatory mRNA expression levels in monocytes and clinical outcome. These data underline the notion that circulating leukocytes are an important source for inflammatory mediators in patients with gram-negative sepsis. Gene profiling such as was done here provides an excellent tool to obtain insight into the extent of inflammation activation in patients with severe infection.
DOI http://dx.doi.org/10.1128/IAI.01733-06   (check subscription with CDU E-Gateway service for CDU Staff and Students  check subscription with CDU E-Gateway in new window)
Additional Notes Copyright by the American Society for Microbiology


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