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Analysis of intragenomic variation of the rDNA internal transcribed spacers (ITS) in Halichondrida (Porifera: Demospongiae)

Alvarez, Belinda, Krishnan, Mahima and Gibb, Karen S. (2007). Analysis of intragenomic variation of the rDNA internal transcribed spacers (ITS) in Halichondrida (Porifera: Demospongiae). The Journal of the Marine Biological Association of the United Kingdom,87(6):1599-1605.

Document type: Journal Article
Citation counts: Scopus Citation Count Cited 11 times in Scopus Article | Citations

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IRMA ID 80802555xPUB9
Title Analysis of intragenomic variation of the rDNA internal transcribed spacers (ITS) in Halichondrida (Porifera: Demospongiae)
Author Alvarez, Belinda
Krishnan, Mahima
Gibb, Karen S.
Journal Name The Journal of the Marine Biological Association of the United Kingdom
Publication Date 2007
Volume Number 87
Issue Number 6
ISSN 0025-3154   (check CDU catalogue open catalogue search in new window)
Scopus ID 2-s2.0-36749077004
Start Page 1599
End Page 1605
Total Pages 7
Place of Publication Cambridge, U.K
Publisher Cambridge University Press
Field of Research 0604 - Genetics
0608 - Zoology
HERDC Category C1 - Journal Article (DEST)
Abstract Three species of sponges, Axinella aruensis, Phakellia sp. and Acanthella cavernosa, were selected to investigate the extent of intragenomic variation (IGV) in the internal transcribed spacers (ITS-1 and ITS-2) of the ribosomal DNA within the order Halichondrida. The IGV was detected in both the spacers and in the three species. At least three non-identical sequences were found in any single individual with the number of polymorphic sites within species ranging from 6 to 77. Uncorrected distance values up to 29% were observed in the ITS-2 of Axinella aruensis. The levels of polymorphism found in this species are the highest reported for Porifera and comparable to those observed in hybrid species of scleractinean corals. Analyses of ITS sequences from multiple clones from individuals of Halichondrida are strongly recommended if these genetic markers are to be used to depict phylogenetic and/or phylogeographic relationships of closely related species and their populations. Data from other nuclear and mitochondrial markers should be used to complement conclusions derived from studies based on ITS sequences.
DOI http://dx.doi.org/10.1017/S0025315407058407   (check subscription with CDU E-Gateway service for CDU Staff and Students  check subscription with CDU E-Gateway in new window)


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