Charles Darwin University

CDU eSpace
Institutional Repository

 
CDU Staff and Student only
 

Hepatic nuclear receptor PPARα in the koala (Phascolarctos cinereus): Cloning and molecular characterisation

Ngo, Suong Ngoc Thi, McKinnon, Ross Allan and Stupans, Ieva (2007). Hepatic nuclear receptor PPARα in the koala (Phascolarctos cinereus): Cloning and molecular characterisation. Comparative Biochemistry and Physiology. Part C: Toxicology & Pharmacology,146(3):375-382.

Document type: Journal Article
Citation counts: Scopus Citation Count Cited 4 times in Scopus Article | Citations

Google Scholar Search Google Scholar

Title Hepatic nuclear receptor PPARα in the koala (Phascolarctos cinereus): Cloning and molecular characterisation
Author Ngo, Suong Ngoc Thi
McKinnon, Ross Allan
Stupans, Ieva
Journal Name Comparative Biochemistry and Physiology. Part C: Toxicology & Pharmacology
Publication Date 2007
Volume Number 146
Issue Number 3
ISSN 1532-0456   (check CDU catalogue open catalogue search in new window)
Scopus ID 2-s2.0-34547234534
Start Page 375
End Page 382
Total Pages 8
Place of Publication United States of America
Publisher Elsevier Inc.
Field of Research 1115 - Pharmacology and Pharmaceutical Sciences
HERDC Category C1 - Journal Article (DEST)
Abstract Peroxisome proliferator-activated receptor α (PPARα) is a member of the nuclear/steroid receptor gene superfamily that plays an essential role in fatty acid metabolism. PPARα modulates the expression of genes encoding peroxisomal fatty acid β-oxidation enzymes and microsomal fatty acid hydroxylases CYP4As. We have previously reported that the obligate Eucalyptus feeder koala (Phascolarctos cinereus) exhibits a higher hepatic CYP4A activity and an absence of peroxisomal palmitoyl-CoA oxidation as compared to non-Eucalyptus feeders human, rat or wallaby. Here we describe the cloning, expression and molecular characterisation of koala hepatic PPARα. A full-length PPARα cDNA of size 1515 bp was cloned by reverse transcription-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE). The koala PPARα cDNA encodes a protein of 468 amino acids. Transfection of the koala PPARα cDNA into Cos-7 cells resulted in the expression of a protein recognised by a rabbit anti-human PPARα polyclonal antibody. PPARα immunoreactive bands of the same molecular mass were detected in nuclear extracts of koala livers. The results of this study demonstrate the presence of koala hepatic PPARα which shares several common features with other published PPARαs; however, it exhibits important differences in both the DNA and ligand binding domains.
Keywords Peroxisome proliferator activated receptor alpha (PPARĪ±)
Eucalyptus terpenes
Reverse transcription polymerase chain reaction (RT-PCR)
Rapid amplification of cDNA end (RACE)
Koala liver
DOI http://dx.doi.org/10.1016/j.cbpc.2007.04.013   (check subscription with CDU E-Gateway service for CDU Staff and Students  check subscription with CDU E-Gateway in new window)
 
Versions
Version Filter Type
Access Statistics: 169 Abstract Views  -  Detailed Statistics
Created: Fri, 12 Sep 2008, 08:35:25 CST by Administrator