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Detection and genetic relatedness of phytoplasmas associated with plant diseases in Thailand

Spoodee, R, Schneider, BL, Padovan, AC and Gibb, KS (1999). Detection and genetic relatedness of phytoplasmas associated with plant diseases in Thailand. Journal of Biochemistry, Molecular Biology with Biophysics,3(2):133-140.

Document type: Journal Article
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Title Detection and genetic relatedness of phytoplasmas associated with plant diseases in Thailand
Author Spoodee, R
Schneider, BL
Padovan, AC
Gibb, KS
Journal Name Journal of Biochemistry, Molecular Biology with Biophysics
Publication Date 1999
Volume Number 3
Issue Number 2
ISSN 1025 8140   (check CDU catalogue open catalogue search in new window)
Start Page 133
End Page 140
Total Pages 8
Place of Publication Malaysia
Publisher Taylor and Francis
HERDC Category C1 - Journal Article (DEST)
Abstract Phytoplasma ribosomal DNA was detected by the polymerase chain reaction (PCR) in diseased plants collected from different parts of Thailand. Among these, bitter gourd phyllody (BGPHY) from Songkhla, South Thailand is a new phytoplasma disease. Restriction fragment length polymorphism (RFLP) patterns from Alu I, Hpa II, Mse I, and Rsa I restriction enzymes indicated 3 phytoplasmas groups. One group contained carpet grass (Axonopus compressus (Sw.) P. Beauv.) white leaf (CGWL), bermuda grass (Cynodon dactylon (L.) Pets.) white leaf (BGWL), sugarcane white leaf(SCWL), and sugarcane green grassy shoot (SCGS). The second group contained sesame phyllody (SESPHY) and sunnhemp phyllody (SUNPHY), and the third group contained periwinkle phyllody (PERPHY) and BGPHY. Only digestion with Mse I permitted differentiation of SCWL from CGWL and BGWL and also from SCGS. Spacer region sequence analysis showed that the 3 groups of phytoplasmas formed distinct phylogenetic clusters. SESPHY was closely related to faba bean phyllody (FABAPHY) while PERPHY and BGPHY were related to the aster yellows agent. Within the white leaf cluster, SCWL and SCGS were more closely related to each other than they were to BGWL and CGWL.
 
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