Charles Darwin University

CDU eSpace
Institutional Repository

 
CDU Staff and Student only
 

Comparison and characterization of tomato big bud- and sweet potato little leaf-group phytoplasmas

Schneider, Bernd L., Gibb, Karen S., Padovan, Anna C., Davis, Richard I. and De La Rue, Stephanie J. (1999). Comparison and characterization of tomato big bud- and sweet potato little leaf-group phytoplasmas. Journal of Phytopathology,147(1):31-40.

Document type: Journal Article
Citation counts:
Google Scholar Search Google Scholar

Title Comparison and characterization of tomato big bud- and sweet potato little leaf-group phytoplasmas
Author Schneider, Bernd L.
Gibb, Karen S.
Padovan, Anna C.
Davis, Richard I.
De La Rue, Stephanie J.
Journal Name Journal of Phytopathology
Publication Date 1999
Volume Number 147
Issue Number 1
ISSN 1439-0434   (check CDU catalogue  open catalogue search in new window)
Start Page 31
End Page 40
Total Pages 10
Place of Publication Berlin
Publisher Blackwell Publishing
HERDC Category C1 - Journal Article (DEST)
Abstract EcoRI or HindIII-restricted DNA from phytoplasmas associated with tomato big bud (TBB) and sweet potato little leaf (SPLL-V4) was cloned into pBluescript and the recombinant plasmids were screened by reciprocal DNA hybridization with TBB- and SPLL-V4 DNA. Most of the recombinant plasmids hybridized to DNA of both phytoplasmas. Southern hybridization analysis of Eco-RI- or HindIII-restricted TBB- and SPLL-V4 DNA with two TBB probes (pTBB32 and pTBB87) gave identical restriction patterns. Southern hybridizations of HindIII-or EcoRI digested DNA from 10 phytoplasma-positive field samples revealed a different restriction fragment pattern in two phytoplasmas from sweet potato. Three TBB probes which did not hybridize to SPLL-V4 were selected for Southern blot analysis of samples. The pTBB42 and pTBB78 hybridized to nine of the 10 TBB-symptomatic field samples producing identical patterns, pTBB88 contained extrachromosomal DNA and hybridized with two phytoplasmas from diseased sweet potato and perennial phlox. The SPLL-V4-specific probe pH 21 hybridized to five field samples generating different restriction patterns. About 80 field samples were subjected to the polymerase chain reaction using primers deduced from pH21, pTBB42 and pTBB88. These phytoplasmas had been previously characterized and grouped by their 16S/23S spacer region restriction fragment pattern but this classification was in most cases different to the results obtained with these primers.
DOI http://dx.doi.org/10.1111/j.1439-0434.1999.tb03804.x   (check subscription with CDU E-Gateway service for CDU Staff and Students  check subscription with CDU E-Gateway in new window)
 
Versions
Version Filter Type
Access Statistics: 78 Abstract Views  -  Detailed Statistics
Created: Fri, 12 Sep 2008, 08:35:25 CST by Administrator