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Usefulness of published PCR primers in detecting human rhinovirus infection

Faux, Cassandra E., Arden, Katherine E., Lambert, Stephen B., Nissen, Michael D., Nolan, Terry M., Chang, Anne B., Sloots, Theo P. and Mackay, Ian M. (2011). Usefulness of published PCR primers in detecting human rhinovirus infection. Emerging Infectious Diseases,17(2):296-298.

Document type: Journal Article
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IRMA ID jsingletonxPUB46
Title Usefulness of published PCR primers in detecting human rhinovirus infection
Author Faux, Cassandra E.
Arden, Katherine E.
Lambert, Stephen B.
Nissen, Michael D.
Nolan, Terry M.
Chang, Anne B.
Sloots, Theo P.
Mackay, Ian M.
Journal Name Emerging Infectious Diseases
Publication Date 2011
Volume Number 17
Issue Number 2
ISSN 1080-6059   (check CDU catalogue  open catalogue search in new window)
Start Page 296
End Page 298
Total Pages 3
Place of Publication United States
Publisher U.S. Department of Health and Human Services, Centers for Disease Control and Prevention
HERDC Category C1 - Journal Article (DEST)
Abstract We conducted a preliminary comparison of the relative sensitivity of a cross-section of published human rhinovirus (HRV)–specific PCR primer pairs, varying the oligonucleotides and annealing temperature. None of the pairs could detect all HRVs in 2 panels of genotyped clinical specimens; >1 PCR is required for accurate description of HRV epidemiology.
Keywords HRV epidemiology
DOI http://dx.doi.org/10.3201/eid1702.101123   (check subscription with CDU E-Gateway service for CDU Staff and Students  check subscription with CDU E-Gateway in new window)


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