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Diversity of 16S-23S rDNA Internal Transcribed Spacer (ITS) reveals phylogenetic relationships in Burkholderia pseudomallei and its near-neighbors

Liguori, Andrew P., Warrington, Stephanie D., Ginther, Jennifer L., Pearson, Talima, Bowers, Jolene, Glass, Mindy B., Mayo, Mark J., Wuthiekanun, Vanaporn, Engelthaler, David, Peacock, Sharon J., Currie, Bart J., Wagner, David M., Keim, Paul and Tuanyok, Apichai (2011). Diversity of 16S-23S rDNA Internal Transcribed Spacer (ITS) reveals phylogenetic relationships in Burkholderia pseudomallei and its near-neighbors. PLoS One,6(12):e29323.

Document type: Journal Article
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Title Diversity of 16S-23S rDNA Internal Transcribed Spacer (ITS) reveals phylogenetic relationships in Burkholderia pseudomallei and its near-neighbors
Author Liguori, Andrew P.
Warrington, Stephanie D.
Ginther, Jennifer L.
Pearson, Talima
Bowers, Jolene
Glass, Mindy B.
Mayo, Mark J.
Wuthiekanun, Vanaporn
Engelthaler, David
Peacock, Sharon J.
Currie, Bart J.
Wagner, David M.
Keim, Paul
Tuanyok, Apichai
Journal Name PLoS One
Publication Date 2011
Volume Number 6
Issue Number 12
ISSN 1932-6203   (check CDU catalogue open catalogue search in new window)
Start Page e29323
Total Pages 8
Place of Publication United States
Publisher Public Library of Science
HERDC Category C1 - Journal Article (DEST)
Abstract Length polymorphisms within the 16S-23S ribosomal DNA internal transcribed spacer (ITS) have been described as stable genetic markers for studying bacterial phylogenetics. In this study, we used these genetic markers to investigate phylogenetic relationships in Burkholderia pseudomallei and its near-relative species. B. pseudomallei is known as one of the most genetically recombined bacterial species. In silico analysis of multiple B. pseudomallei genomes revealed approximately four homologous rRNA operons and ITS length polymorphisms therein. We characterized ITS distribution using PCR and analyzed via a high-throughput capillary electrophoresis in 1,191 B. pseudomallei strains. Three major ITS types were identified, two of which were commonly found in most B. pseudomallei strains from the endemic areas, whereas the third one was significantly correlated with worldwide sporadic strains. Interestingly, mixtures of the two common ITS types were observed within the same strains, and at a greater incidence in Thailand than Australia suggesting that genetic recombination causes the ITS variation within species, with greater recombination frequency in Thailand. In addition, the B. mallei ITS type was common to B. pseudomallei, providing further support that B. mallei is a clone of B. pseudomallei. Other B. pseudomallei near-neighbors possessed unique and monomorphic ITS types. Our data shed light on evolutionary patterns of B. pseudomallei and its near relative species.
DOI http://dx.doi.org/10.1371/journal.pone.0029323   (check subscription with CDU E-Gateway service for CDU Staff and Students  check subscription with CDU E-Gateway in new window)


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