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Generation and characterization of cDNA clones from Sarcoptes scabiei var. hominis for an expressed sequence tag library: identification of homologues of house dust mite allergens

Fischer, Katja, Holt, Deborah C., Harumal, Pearly, Currie, Bart J., Walton, Shelley F. and Kemp, David J. (2003). Generation and characterization of cDNA clones from Sarcoptes scabiei var. hominis for an expressed sequence tag library: identification of homologues of house dust mite allergens. American Journal of Tropical Medicine and Hygiene,68(1):61-64.

Document type: Journal Article
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Title Generation and characterization of cDNA clones from Sarcoptes scabiei var. hominis for an expressed sequence tag library: identification of homologues of house dust mite allergens
Author Fischer, Katja
Holt, Deborah C.
Harumal, Pearly
Currie, Bart J.
Walton, Shelley F.
Kemp, David J.
Journal Name American Journal of Tropical Medicine and Hygiene
Publication Date 2003
Volume Number 68
Issue Number 1
ISSN 0002-9637   (check CDU catalogue open catalogue search in new window)
Start Page 61
End Page 64
Total Pages 4
Publisher The American Society of Tropical Medicine and Hygiene
Field of Research 1117 - Public Health and Health Services
HERDC Category C1 - Journal Article (DEST)
Abstract Molecular studies on scabies, a disease of considerable human and veterinary significance, have been limited because of the difficulty of obtaining the causative organism Sarcoptes scabiei, the "itch mite." We have used skin from the bedding of crusted scabies patients as a source of mites for the construction of libraries of cDNAs from S. scabiei var. hominis in the bacteriophage {lambda} vector {lambda}ZAP express. Sequences of 145 clones established that the libraries predominantly contain sequences from S. scabiei, enabling a major sequencing program to begin. Among those sequenced to date, cDNAs encoding S. scabiei homologues of 3 house dust mite allergens—the M-177 apolipoprotein, glutathione S-transferase, and paramyosin—were identified. The availability of cDNA libraries from S. scabiei var. hominis and S. scabiei var. vulpes and the emerging public sequence databases from both opens up new possibilities in scabies research.
Keywords Allergens
animal
Base Sequence
chemistry
Cloning,Molecular
Disease
DNA,Complementary
Expressed Sequence Tags
genetics
immunology
molecular
Molecular Sequence Data
Pyroglyphidae
Sarcoptes scabiei
Scabies
Sequence Alignment
Sequence Homology
skin
 
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Created: Mon, 26 Nov 2007, 15:07:47 CST