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Prime boost vaccination strategies: CD8 T cell numbers, protection, and Th1 bias

Woodberry, Tonia, Gardner, Joy, Elliott, Suzanne L., Leyrer, Sonja, Purdie, David M., Chaplin, Paul and Suhrbier, Andreas (2003). Prime boost vaccination strategies: CD8 T cell numbers, protection, and Th1 bias. Journal of Immunology,170(5):2599-2604.

Document type: Journal Article
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Title Prime boost vaccination strategies: CD8 T cell numbers, protection, and Th1 bias
Author Woodberry, Tonia
Gardner, Joy
Elliott, Suzanne L.
Leyrer, Sonja
Purdie, David M.
Chaplin, Paul
Suhrbier, Andreas
Journal Name Journal of Immunology
Publication Date 2003
Volume Number 170
Issue Number 5
ISSN 0022-1767   (check CDU catalogue open catalogue search in new window)
Start Page 2599
End Page 2604
Total Pages 6
Place of Publication Bethesda, MD
Publisher American Association of Immunologists
Field of Research 1107 - Immunology
HERDC Category C1 - Journal Article (DEST)
Abstract Vaccination strategies involving priming with DNA and boosting with a poxvirus vector have emerged as a preferred combination for the induction of protective CD8 T cell immunity. Using IFN-gamma ELISPOT and a series of DNA plasmid, peptide, and modified vaccinia Ankara (MVA) vaccine combinations, we demonstrate that the DNA/MVA combination was uniquely able to enhance IFN-gamma secretion by Ag-specific CD8 T cells. However, CD8 T cell populations induced by DNA/MVA vaccination failed to show an enhanced capability to mediate protection in an IFN-gamma-independent influenza challenge model. The DNA/MVA vaccine strategy was also not unique in its ability to induce high numbers of CD8 T cells, with optimal strategies simply requiring the use of vaccine modalities that individually induce high numbers of CD8 T cells. These experiments argue that rivals to DNA/poxvirus vaccination strategies for the induction of optimal protective CD8 T cell responses are likely to emerge
Keywords Animals
CD8-Positive T-Lymphocytes
Genetic Vectors
Influenza A virus
Influenza Vaccines
Interferon Type II
Lymphocyte Activation
Lymphocyte Count
Th1 Cells
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Created: Mon, 26 Nov 2007, 15:07:47 CST