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Stimulation of microbial sulphate reduction in a constructed wetland: microbiological and geochemical analysis

Lloyd, J. R., Klessa, D. A., Parry, David L., Buck, Paul and Brown, N. L. (2004). Stimulation of microbial sulphate reduction in a constructed wetland: microbiological and geochemical analysis. Water Research,38(7):1822-1830.

Document type: Journal Article
Citation counts: Scopus Citation Count Cited 27 times in Scopus Article | Citations

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Title Stimulation of microbial sulphate reduction in a constructed wetland: microbiological and geochemical analysis
Author Lloyd, J. R.
Klessa, D. A.
Parry, David L.
Buck, Paul
Brown, N. L.
Journal Name Water Research
Publication Date 2004
Volume Number 38
Issue Number 7
ISSN 0043-1354   (check CDU catalogue open catalogue search in new window)
Scopus ID 2-s2.0-1542722941
Start Page 1822
End Page 1830
Total Pages 9
Place of Publication Oxford
Publisher Elsevier Science
Field of Research 0799 - Other Agricultural and Veterinary Sciences
0907 - Environmental Engineering
HERDC Category C1 - Journal Article (DEST)
Abstract Microbial sulphate reduction was stimulated successfully in enclosures installed in a constructed wetland. When sucrose (2.4 mM) and NH40 (600 muM) were added to water in the test enclosures, the indigenous microbial community was able to remove over 90% of the sulphate, present as a contaminant from nearby mining activity at a concentration of 384mgl(-1) (4mM), over 50 days. Over 90% of the sucrose was also removed. Sulphate was not reduced in control enclosures containing no added sucrose or NH4Cl. Fermentation of sucrose by obligate anaerobes including Clostridium sp. and Bacteriodes sp. preceded sulphate reduction in the test enclosures. Sulphate reduction was biphasic, with maximum rates noted between 2-5 and 23-27 days after the addition of the growth substrates. Relatively unbiased 16S rDNA analysis suggested that nitrogen-fixing bacteria were important constituents of the microbial community in the test enclosures at day 23, suggesting that soluble nitrogen was limiting in the amended test enclosures during the experiment.
Keywords sulphate-reducing bacteria
molecular ecology
16s rrna analysis
bioremediation
mine drainage
metals
genes
acid
DOI http://dx.doi.org/10.1016/j.watres.2003.12.033   (check subscription with CDU E-Gateway service for CDU Staff and Students  check subscription with CDU E-Gateway in new window)
 
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