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Sequence characterization of four putative membrane-associated proteins from sweet potato little leaf strain V4 phytoplasma

Mounsey, Kate E., Streten-Joyce, Claire and Gibb, Karen S. (2006). Sequence characterization of four putative membrane-associated proteins from sweet potato little leaf strain V4 phytoplasma. Plant Pathology,55(1):29-35.

Document type: Journal Article
Citation counts: Scopus Citation Count Cited 4 times in Scopus Article | Citations

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IRMA ID 10245xPUB1
Title Sequence characterization of four putative membrane-associated proteins from sweet potato little leaf strain V4 phytoplasma
Author Mounsey, Kate E.
Streten-Joyce, Claire
Gibb, Karen S.
Journal Name Plant Pathology
Publication Date 2006
Volume Number 55
Issue Number 1
ISSN 1365-3059   (check CDU catalogue open catalogue search in new window)
Scopus ID 2-s2.0-33645235458
Start Page 29
End Page 35
Total Pages 7
Publisher Blackwell Publishing
Field of Research 0605 - Microbiology
0607 - Plant Biology
HERDC Category C1 - Journal Article (DEST)
Abstract To increase understanding of the genetic architecture of phytoplasmas, a random clone (pH80) of the sweet potato little leaf strain-V4 (SPLL-V4) phytoplasma and the glucose-inhibited division protein A (gidA) gene were analysed. Clone pH80 and the gidA gene are located in a 100-kb region near the putative origin of replication. Three ORFs were identified within the pH80 random clone, encoding the proteins PotB, PotC and PotD, which form part of an ABC transport system for polyamine import. Sequence data were supported by structural analysis which indicated that SPLL-V4 PotB and PotC were integral membrane proteins, and PotD was a substrate-binding, surface-exposed membrane protein. The gidA gene in the SPLL-V4 phytoplasma was also putatively identified as a membrane protein. The complete gidA gene sequences were characterized for the tomato big bud and SPLL-V4 phytoplasmas. The proteins identified here were found to share high similarity with those recently identified in the onion yellows phytoplasma genome. Both the gidA and polyamine transport operons are believed to be associated with DNA replication.
Keywords abc transporter
phytoplasma membrane proteins
pot gene
apple proliferation phytoplasma
tomato big bud
physical map
chromosome
gene
genome
identification
prediction
mycoplasma
evolution
DOI http://dx.doi.org/10.1111/j.1365-3059.2005.01291.x   (check subscription with CDU E-Gateway service for CDU Staff and Students  check subscription with CDU E-Gateway in new window)
 
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Created: Wed, 28 Nov 2007, 14:16:08 CST