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Proteases of liver fluke - enzymatic characterisation and prospects for vaccination against fasciolosis

Norbury, Luke, Jayaraj, Rama, Pike, Robert, Spithill, Terry W. and Smooker, Peter M. (2010). Proteases of liver fluke - enzymatic characterisation and prospects for vaccination against fasciolosis. In: Baby, Saji, Singh Sandhu, Parvinder and Hang, Yi 2010 International Conference on Agriculture and Animal Science, Singapore, 26-28 Februar 2010.

Document type: Conference Paper

IRMA ID 83093774xPUB202
Author Norbury, Luke
Jayaraj, Rama
Pike, Robert
Spithill, Terry W.
Smooker, Peter M.
Title Proteases of liver fluke - enzymatic characterisation and prospects for vaccination against fasciolosis
Conference Name 2010 International Conference on Agriculture and Animal Science
Conference Location Singapore
Conference Dates 26-28 Februar 2010
Conference Publication Title Proceedings of 2010 international conference on Agriculture and Animal Science (CAAS 2010)
Editor Baby, Saji
Singh Sandhu, Parvinder
Hang, Yi
Place of Publication Liverpool, United Kingdom
Publisher World Academic Union (World Academic Press)
Publication Year 2010
ISBN 978-1-84626-022-3   (check CDU catalogue open catalogue search in new window)
Total Pages 5
HERDC Category E1 - Conference Publication (DIISR)
Abstract Liver flukes produce cathepsin B and cathepsin L in their excretory-secretory material. Cathepsin B is predominately released in the juvenile stage of the life-cycle, while different cathepsin L's are released throughout the cycle. Phylogenetic analysis of the mature proteins shows that cathepsin L's and B form distinct clades, as expected. Cathepsin L proteases also form clades that reflect their predicted enzymatic activity, and their temporal expression. We have expressed examples of both these cathepsins in yeast, and purified them for both enzymatic analysis and for use as vaccines. Recently, a vaccination experiment was performed combining cathepsins B and L as immunogens, demonstrating that a multivalent vaccine may be optimal.
Keyword Cathepsins
Fasciola
Vaccine
 
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Created: Fri, 17 Jan 2014, 00:13:40 CST