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Genetic diversity of common carp in Vietnam using direct sequencing and SSCP analysis of the mitochondrial DNA control region

Thai, B., Pham, Thuy Thu and Austin, Christopher M. (2006). Genetic diversity of common carp in Vietnam using direct sequencing and SSCP analysis of the mitochondrial DNA control region. Aquaculture,258(1-Apr):228-240.

Document type: Journal Article
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IRMA ID 78674128xPUB9
Title Genetic diversity of common carp in Vietnam using direct sequencing and SSCP analysis of the mitochondrial DNA control region
Author Thai, B.
Pham, Thuy Thu
Austin, Christopher M.
Journal Name Aquaculture
Publication Date 2006
Volume Number 258
Issue Number 1-Apr
ISSN 1873-5622   (check CDU catalogue open catalogue search in new window)
Start Page 228
End Page 240
Total Pages 13
Place of Publication Amsterdam
Publisher Elsevier
Field of Research 0603 - Evolutionary Biology
0704 - Fisheries Sciences
HERDC Category C1 - Journal Article (DEST)
Abstract The aquaculture of common carp (Cyprinus carpio L.) in Vietnam is based upon both indigenous and introduced stocks which have not been subject to study using modem molecular genetic methods to any significant extent. Twenty strains or populations of common carp in Vietnam represented by 968 fish were screened for variation in mitochondrial control region fragments using a combination of direct DNA sequencing and Single Strand Confirmation Polymorphism (SSCP) analysis. Common carp samples from China, Japan, Indonesia, and Hungary were analysed for comparison. Sequencing revealed that Vietnamese common carp have high haplotype, but low nucleotide diversity and represent a mixture of indigenous and introduced strains. The SSCP procedure resolved eight haplotypes that distinguished Indonesian, Hungarian and Vietnamese strains and which varied significantly among hatchery and wild Vietnamese common carp populations. Intra-population diversity was least in experimental common carp lines, maintained at the Research Institute for Aquaculture No 1 and greatest within hatchery stocks. Relationships among populations based on SSCP haplotype frequencies showed that the RIA1 Vietnamese white common carp strain is closely related to wild populations, which in turn are closely related to six of the eleven hatchery stocks. The other five hatchery stocks had higher levels of interpopulation variation and mostly showed a closer relationship to Indonesian yellow carp strains. The Hungarian carp strain was highly divergent from all other populations suggesting that this strain has not contributed significantly to the establishment of cultured stocks in Vietnam, based on this maternally inherited marker. The SSCP procedure shows considerable potential for rapid genotyping and genetic characterization of common carp and therefore, for investigation of diversity in wild stocks and broodstock management.
Keywords genetic diversity
vietnam
cyprinus carpio
control region
sequencing
catfish ictalurus-punctatus
cyprinus-carpio
phylogenetic-relationships
populations
microsatellite markers
south-american
variability
strains
DOI http://dx.doi.org/10.1016/j.aquaculture.2006.03.025   (check subscription with CDU E-Gateway service for CDU Staff and Students  check subscription with CDU E-Gateway in new window)
 
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