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Plasmodium vivax: Isotopic, PicoGreen, and microscopic assays for measuring chloroquine sensitivity in fresh and cryopreserved isolates

Kosaisavee, V, Suwanarusk, R, Nosten, F, Kyle, D, Barrends, M, Jones, J, Price, Ric, Russell, B and Lek-uthai, U (2006). Plasmodium vivax: Isotopic, PicoGreen, and microscopic assays for measuring chloroquine sensitivity in fresh and cryopreserved isolates. Experimental Parasitology,114(1):34-39.

Document type: Journal Article
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IRMA ID 10202xPUB35
Title Plasmodium vivax: Isotopic, PicoGreen, and microscopic assays for measuring chloroquine sensitivity in fresh and cryopreserved isolates
Author Kosaisavee, V
Suwanarusk, R
Nosten, F
Kyle, D
Barrends, M
Jones, J
Price, Ric
Russell, B
Lek-uthai, U
Journal Name Experimental Parasitology
Publication Date 2006
Volume Number 114
Issue Number 1
ISSN 0014-4894   (check CDU catalogue open catalogue search in new window)
Start Page 34
End Page 39
Total Pages 6
Place of Publication US
Publisher Academic Press
Field of Research 0605 - Microbiology
0707 - Veterinary Sciences
HERDC Category C1 - Journal Article (DEST)
Abstract In vitro susceptibility tests provide information on the intrinsic response of Plasmodium vivax to antimalarials, free from confounding factors such as host immunity or relapse. This study examined the utility of radioisotope and PicoGreen assays as alternatives to the traditional microscopic examination for assessing response of P. vivax to antimalarial drugs. There was no significant difference in the mean chloroquine IC50 of P. vivax (n = 40) as determined by the microscopic (33.4 ng/ml), isotopic (33.6 ng/ml), and PicoGreen (39.1 ng/ml) assays, respectively (F = 0.239, df = 2, 51, and p = 0.788). However measurement of IC50s by the microscopic method was slightly more successful in producing valid assays (57%), compared to the isotopic (32.5%) and PicoGreen (45.5%) methods. In a paired comparison of 20 fresh and cryopreserved isolates as examined by the microscopic method, there were no significant differences between the mean IC50 responses (T = 1.58, df = 15, and p = 0.34). Detailed methodologies for the short time culture of field and cryopreserved P. vivax are described. Although the microscopic in vitro assay provides a useful method for characterizing the drug susceptibility phenotype of P. vivax isolates, its utility is limited by a laborious methodology and need for highly skilled microscopists. Future efforts should focus on further development of high throughput assays such as the PicoGreen assay as described in this study.
Keywords Plasmodium vivax
Chloroquine
Antimalarial sensitivity
In vitro
Isotopic
PicoGreen
Microscopic
Cryopreservation
DOI http://dx.doi.org/10.1016/j.exppara.2006.02.006   (check subscription with CDU E-Gateway service for CDU Staff and Students  check subscription with CDU E-Gateway in new window)
 
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Created: Wed, 28 Nov 2007, 14:16:08 CST