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Results from an inter-laboratory comparison of pneumococcal serotype-specific IgG measurement and critical parameters that affect assay performance

Balloch, Anne, Licciardi, Paul V., Leach, Amanda J., Nurkka, A. and Tang, M. L. K. (2010). Results from an inter-laboratory comparison of pneumococcal serotype-specific IgG measurement and critical parameters that affect assay performance. Vaccine,28(5):1333-1340.

Document type: Journal Article

IRMA ID 75039815xPUB181
Title Results from an inter-laboratory comparison of pneumococcal serotype-specific IgG measurement and critical parameters that affect assay performance
Author Balloch, Anne
Licciardi, Paul V.
Leach, Amanda J.
Nurkka, A.
Tang, M. L. K.
Journal Name Vaccine
Publication Date 2010
Volume Number 28
Issue Number 5
ISSN 0264-410X   (check CDU catalogue open catalogue search in new window)
Start Page 1333
End Page 1340
Total Pages 8
Place of Publication United Kingdom
Publisher Elsevier Ltd
HERDC Category C1 - Journal Article (DIISR)
Abstract Quantitation of specific IgG to polysaccharides (serotypes) of Streptococcus pneumoniae provides the basis for evaluating vaccine efficacy. Different enzyme-linked immunosorbent assay (ELISA) methods are used internationally, making comparisons between laboratories difficult. We undertook an inter-laboratory comparison between two international laboratories performing serotype-specific IgG ELISAs using a panel of well-characterized serum samples: the Murdoch Childrens Research Institute Pneumococcal Laboratory (Melbourne, Australia) and the Vaccine Immunology Laboratory, National Public Health Institute (Helsinki, Finland). While good agreement was found for the inter-laboratory comparison for most serotypes, differences in ELISA methodology influenced specific IgG measurement. Therefore, use of the World Health Organization (WHO)-based ELISA methods for measurement of serotype-specific IgG is reliable, accurate and provides consistent results between international laboratories.
Keywords Pneumococcal
IgG
ELISA
DOI http://dx.doi.org/10.1016/j.vaccine.2009.11.011   (check subscription with CDU E-Gateway service for CDU Staff and Students  check subscription with CDU E-Gateway in new window)
 
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Created: Fri, 17 Jan 2014, 00:27:58 CST