Charles Darwin University

CDU eSpace
Institutional Repository

 
CDU Staff and Student only
 

BurkDiff: a real-time PCR allelic discriminiation assay for Burkholderia pseudomallei and B. mallei

Bowers, Jolene R., Engelthaler, David M., Ginther, Jennifer L., Pearson, Talima, Peacock, Sharon J., Tuanyok, Apichai, Wagner, David M., Currie, Bart J. and Keim, Paul (2010). BurkDiff: a real-time PCR allelic discriminiation assay for Burkholderia pseudomallei and B. mallei. PLoS One,5(11):e15413.

Document type: Journal Article
Attached Files (Some files may be inaccessible until you login with your CDU eSpace credentials)
Name Description MIMEType Size Downloads
Download this reading Currie_37327.pdf Published version application/pdf 448.60KB 66
Reading the attached file works best in Firefox, Chrome and IE 9 or later.

IRMA ID 81704288xPUB265
Title BurkDiff: a real-time PCR allelic discriminiation assay for Burkholderia pseudomallei and B. mallei
Author Bowers, Jolene R.
Engelthaler, David M.
Ginther, Jennifer L.
Pearson, Talima
Peacock, Sharon J.
Tuanyok, Apichai
Wagner, David M.
Currie, Bart J.
Keim, Paul
Journal Name PLoS One
Publication Date 2010
Volume Number 5
Issue Number 11
ISSN 1932-6203   (check CDU catalogue open catalogue search in new window)
Start Page e15413
Total Pages 7
Place of Publication United States
Publisher Public Library of Science
HERDC Category C1 - Journal Article (DIISR)
Abstract A real-time PCR assay, BurkDiff, was designed to target a unique conserved region in the B. pseudomallei and B. mallei genomes containing a SNP that differentiates the two species. Sensitivity and specificity were assessed by screening BurkDiff across 469 isolates of B. pseudomallei, 49 isolates of B. mallei, and 390 isolates of clinically relevant non-target species. Concordance of results with traditional speciation methods and no cross-reactivity to non-target species show BurkDiff is a robust, highly validated assay for the detection and differentiation of B. pseudomallei and B. mallei.
DOI http://dx.doi.org/10.1371/journal.pone.0015413   (check subscription with CDU E-Gateway service for CDU Staff and Students  check subscription with CDU E-Gateway in new window)


© copyright

Every reasonable effort has been made to ensure that permission has been obtained for items included in CDU eSpace. If you believe that your rights have been infringed by this repository, please contact digitisation@cdu.edu.au.

 
Versions
Version Filter Type
Access Statistics: 55 Abstract Views, 66 File Downloads  -  Detailed Statistics
Created: Fri, 17 Jan 2014, 00:28:45 CST