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BurkDiff: a real-time PCR allelic discriminiation assay for Burkholderia pseudomallei and B. mallei

Bowers, Jolene R., Engelthaler, David M., Ginther, Jennifer L., Pearson, Talima, Peacock, Sharon J., Tuanyok, Apichai, Wagner, David M., Currie, Bart J. and Keim, Paul (2010). BurkDiff: a real-time PCR allelic discriminiation assay for Burkholderia pseudomallei and B. mallei. PLoS One,5(11):e15413.

Document type: Journal Article
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IRMA ID 81704288xPUB265
Title BurkDiff: a real-time PCR allelic discriminiation assay for Burkholderia pseudomallei and B. mallei
Author Bowers, Jolene R.
Engelthaler, David M.
Ginther, Jennifer L.
Pearson, Talima
Peacock, Sharon J.
Tuanyok, Apichai
Wagner, David M.
Currie, Bart J.
Keim, Paul
Journal Name PLoS One
Publication Date 2010
Volume Number 5
Issue Number 11
ISSN 1932-6203   (check CDU catalogue  open catalogue search in new window)
Start Page e15413
Total Pages 7
Place of Publication United States
Publisher Public Library of Science
HERDC Category C1 - Journal Article (DIISR)
Abstract A real-time PCR assay, BurkDiff, was designed to target a unique conserved region in the B. pseudomallei and B. mallei genomes containing a SNP that differentiates the two species. Sensitivity and specificity were assessed by screening BurkDiff across 469 isolates of B. pseudomallei, 49 isolates of B. mallei, and 390 isolates of clinically relevant non-target species. Concordance of results with traditional speciation methods and no cross-reactivity to non-target species show BurkDiff is a robust, highly validated assay for the detection and differentiation of B. pseudomallei and B. mallei.
DOI http://dx.doi.org/10.1371/journal.pone.0015413   (check subscription with CDU E-Gateway service for CDU Staff and Students  check subscription with CDU E-Gateway in new window)


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