Charles Darwin University

CDU eSpace
Institutional Repository

 
CDU Staff and Student only
 

The non-serotypeable pneumococcus: phenotypic dynamics in the era of anti-capsular vaccines

Marsh, Robyn L., Smith-Vaughan, Heidi C., Hare, Kim M., Binks, Michael J., Kong, Fanrong, Warning, Julia, Gilbert, Gwendolyn, Morris, Peter S. and Leach, Amanda J. (2010). The non-serotypeable pneumococcus: phenotypic dynamics in the era of anti-capsular vaccines. Journal of Clinical Microbiology,48(3):831-835.

Document type: Journal Article
Attached Files (Some files may be inaccessible until you login with your CDU eSpace credentials)
Name Description MIMEType Size Downloads
Download this reading Marsh_37375.pdf Published version application/pdf 138.73KB 44
Reading the attached file works best in Firefox, Chrome and IE 9 or later.

IRMA ID 81704288xPUB315
Title The non-serotypeable pneumococcus: phenotypic dynamics in the era of anti-capsular vaccines
Author Marsh, Robyn L.
Smith-Vaughan, Heidi C.
Hare, Kim M.
Binks, Michael J.
Kong, Fanrong
Warning, Julia
Gilbert, Gwendolyn
Morris, Peter S.
Leach, Amanda J.
Journal Name Journal of Clinical Microbiology
Publication Date 2010
Volume Number 48
Issue Number 3
ISSN 0095-1137   (check CDU catalogue  open catalogue search in new window)
Start Page 831
End Page 835
Total Pages 5
Place of Publication Washington, United States
Publisher American Society for Microbiology
HERDC Category C1 - Journal Article (DIISR)
Abstract Nonserotypeable pneumococci (NSP) are commonly carried by Australian Indigenous children in remote communities. The purpose of this study was to characterize carriage isolates of NSP from Indigenous children vaccinated with the seven-valent pneumococcal conjugate vaccine (PCV7) and to use these data to guide decisions on reporting of NSP. A total of 182 NSP were characterized by BOX typing, antibiogram analysis, and multilocus sequence typing (MLST) of common BOX types. NSP positive for the wzg capsule gene were analyzed by a multiplex PCR-based reverse line blot hybridization assay (mPCR/RLB-H) targeting capsule genes to determine the serotype. Among 182 NSP, 49 BOX types were identified. MLST of 10 representative isolates found 7 STs, including ST448 (which accounted for 11% of NSP). Non-penicillin susceptibility was evident in 51% of the isolates. Pneumococcal wzg sequences were detected in only 23 (13%) NSP, including 10 that contained an ∼1.2-kb insert in the region. mPCR/RLB-H identified serotype 14 wzy sequences in all 10 NSP, and 1 also contained a serotype 3-specific wze sequence. Among the remaining 13 wzg-positive NSP, few belonged to the serotypes represented in PCV7. It appears that most NSP identified in Australian Indigenous children are from a true nonencapsulated lineage. Few NSP represented serotypes in PCV7 that suppress capsular expression. High rates of carriage and penicillin resistance and the occasional presence of capsule genes suggest a role for NSP in the maintenance and survival of capsulated pneumococci. To avoid the inflation of pneumococcal carriage and antibiotic resistance rates, in clinical trials, we recommend separate reporting of rates of capsular strains and NSP and the exclusion of data for NSP from primary analyses.


DOI http://dx.doi.org/10.1128/JCM.01701-09   (check subscription with CDU E-Gateway service for CDU Staff and Students  check subscription with CDU E-Gateway in new window)


© copyright

Every reasonable effort has been made to ensure that permission has been obtained for items included in CDU eSpace. If you believe that your rights have been infringed by this repository, please contact digitisation@cdu.edu.au.

 
Versions
Version Filter Type
Access Statistics: 42 Abstract Views, 44 File Downloads  -  Detailed Statistics
Created: Fri, 17 Jan 2014, 00:31:29 CST