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Microbiological applications of high-resolution melting analysis

Tong, Steven Y. C. and Giffard, Philip M. (2012). Microbiological applications of high-resolution melting analysis. Journal of Clinical Microbiology,50(11):3418-3421.

Document type: Journal Article
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IRMA ID bsmithxPUB116
Title Microbiological applications of high-resolution melting analysis
Author Tong, Steven Y. C.
Giffard, Philip M.
Journal Name Journal of Clinical Microbiology
Publication Date 2012
Volume Number 50
Issue Number 11
ISSN 0095-1137   (check CDU catalogue open catalogue search in new window)
Scopus ID 2-s2.0-84867493781
Start Page 3418
End Page 3421
Total Pages 4
Place of Publication Washington, United States
Publisher American Society for Microbiology
HERDC Category C1 - Journal Article (DIISR)
Abstract High-resolution melting (HRM) analysis uses real-time PCR instrumentation to interrogate DNA sequence variation and is a low-cost, single-step, closed-tube method. Here we describe HRM technology and provide examples of varied clinical microbiological applications to highlight the strengths and limitations of HRM analysis.

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