Charles Darwin University

CDU eSpace
Institutional Repository

 
CDU Staff and Student only
 

Detection of Burkholderia pseudomallei O-antigen serotypes in near-neighbor species

Stone, Joshua K., Mayo, Mark J., Grasso, Stephanie A., Ginther, Jennifer L., Warrington, Stephanie D., Allender, Christopher J., Doyle, Adina, Georgia, Shalamar, Kaestli, Mirjam E., Broomall, Stacey M., Karavis, Mark A., Insalaco, Joseph M., Hubbard, Kyle S., McNew, Lauren A., Gibbons, Henry S., Currie, Bart J., Keim, Paul and Tuanyok, Apichai (2012). Detection of Burkholderia pseudomallei O-antigen serotypes in near-neighbor species. BMC Microbiology,12:1-8.

Document type: Journal Article
Attached Files (Some files may be inaccessible until you login with your CDU eSpace credentials)
Name Description MIMEType Size Downloads
Download this reading Mayo_38317.pdf Published version application/pdf 1.38MB 43
Reading the attached file works best in Firefox, Chrome and IE 9 or later.

IRMA ID bsmithxPUB149
Title Detection of Burkholderia pseudomallei O-antigen serotypes in near-neighbor species
Author Stone, Joshua K.
Mayo, Mark J.
Grasso, Stephanie A.
Ginther, Jennifer L.
Warrington, Stephanie D.
Allender, Christopher J.
Doyle, Adina
Georgia, Shalamar
Kaestli, Mirjam E.
Broomall, Stacey M.
Karavis, Mark A.
Insalaco, Joseph M.
Hubbard, Kyle S.
McNew, Lauren A.
Gibbons, Henry S.
Currie, Bart J.
Keim, Paul
Tuanyok, Apichai
Journal Name BMC Microbiology
Publication Date 2012
Volume Number 12
ISSN 1471-2180   (check CDU catalogue open catalogue search in new window)
Scopus ID 2-s2.0-84868239952
Start Page 1
End Page 8
Total Pages 7
Place of Publication United Kingdom
Publisher BioMed Central Ltd.
HERDC Category C1 - Journal Article (DIISR)
Abstract Background
Burkholderia pseudomallei is the etiological agent of melioidosis and a CDC category B select agent with no available effective vaccine. Previous immunizations in mice have utilized the lipopolysaccharide (LPS) as a potential vaccine target because it is known as one of the most important antigenic epitopes in B. pseudomallei. Complicating this strategy are the four different B. pseudomallei LPS O-antigen types: A, B, B2, and rough. Sero-crossreactivity is common among O-antigens of Burkholderia species. Here, we identified the presence of multiple B. pseudomallei O-antigen types and sero-crossreactivity in its near-neighbor species.

Results
PCR screening of O-antigen biosynthesis genes, phenotypic characterization using SDS-PAGE, and immunoblot analysis showed that majority of B. mallei and B. thailandensis strains contained the typical O-antigen type A. In contrast, most of B. ubonensis and B. thailandensis-like strains expressed the atypical O-antigen types B and B2, respectively. Most B. oklahomensis strains expressed a distinct and non-seroreactive O-antigen type, except strain E0147 which expressed O-antigen type A. O-antigen type B2 was also detected in B. thailandensis 82172, B. ubonensis MSMB108, and Burkholderia sp. MSMB175. Interestingly, B. thailandensis-like MSMB43 contained a novel serotype B positive O-antigen.

Conclusions
This study expands the number of species which express B. pseudomallei O-antigen types. Further work is required to elucidate the full structures and how closely these are to the B. pseudomallei O-antigens, which will ultimately determine the efficacy of the near-neighbor B serotypes for vaccine development.
DOI http://dx.doi.org/10.1186/1471-2180-12-250   (check subscription with CDU E-Gateway service for CDU Staff and Students  check subscription with CDU E-Gateway in new window)


© copyright

Every reasonable effort has been made to ensure that permission has been obtained for items included in CDU eSpace. If you believe that your rights have been infringed by this repository, please contact digitisation@cdu.edu.au.

 
Versions
Version Filter Type
Access Statistics: 35 Abstract Views, 43 File Downloads  -  Detailed Statistics
Created: Fri, 17 Jan 2014, 01:27:31 CST