Charles Darwin University

CDU eSpace
Institutional Repository

 
CDU Staff and Student only
 

Quantitative PCR-based genome size estimation of the astimatid mites Sarcoptes scabiei, Psoroptes ovis and Dermatophagoides pteronyssinus

Mounsey, Kate E., Willis, Charlene, Burgess, Stewart T. G., Holt, Deborah C., McCarthy, James and Fischer, Katja (2012). Quantitative PCR-based genome size estimation of the astimatid mites Sarcoptes scabiei, Psoroptes ovis and Dermatophagoides pteronyssinus. Parasites and Vectors,5(3):1-7.

Document type: Journal Article
Attached Files (Some files may be inaccessible until you login with your CDU eSpace credentials)
Name Description MIMEType Size Downloads
Download this reading Holt_38337.pdf Published version application/pdf 347.00KB 36
Reading the attached file works best in Firefox, Chrome and IE 9 or later.

IRMA ID bsmithxPUB78
Title Quantitative PCR-based genome size estimation of the astimatid mites Sarcoptes scabiei, Psoroptes ovis and Dermatophagoides pteronyssinus
Author Mounsey, Kate E.
Willis, Charlene
Burgess, Stewart T. G.
Holt, Deborah C.
McCarthy, James
Fischer, Katja
Journal Name Parasites and Vectors
Publication Date 2012
Volume Number 5
Issue Number 3
ISSN 1756-3305   (check CDU catalogue  open catalogue search in new window)
Scopus ID 2-s2.0-84855283969
Start Page 1
End Page 7
Total Pages 7
Place of Publication United Kingdom
Publisher BioMed Central
HERDC Category C1 - Journal Article (DIISR)
Abstract

Background:
The lack of genomic data available for mites limits our understanding of their biology. Evolving highthroughput
sequencing technologies promise to deliver rapid advances in this area, however, estimates of genome
size are initially required to ensure sufficient coverage.

Methods:

Quantitative real-time PCR was used to estimate the genome sizes of the burrowing ectoparasitic mite
Sarcoptes scabiei, the non-burrowing ectoparasitic mite Psoroptes ovis, and the free-living house dust mite
Dermatophagoides pteronyssinus. Additionally, the chromosome number of S. scabiei was determined by
chromosomal spreads of embryonic cells derived from single eggs.

Results:

S. scabiei cells were shown to contain 17 or 18 small (< 2 μM) chromosomes, suggesting an XO sexdetermination
mechanism. The average estimated genome sizes of S. scabiei and P. ovis were 96 (± 7) Mb and 86
(± 2) Mb respectively, among the smallest arthropod genomes reported to date. The D. pteronyssinus genome was
estimated to be larger than its parasitic counterparts, at 151 Mb in female mites and 218 Mb in male mites.

Conclusions:

This data provides a starting point for understanding the genetic organisation and evolution of these
astigmatid mites, informing future sequencing projects. A comparitive genomic approach including these three
closely related mites is likely to reveal key insights on mite biology, parasitic adaptations and immune evasion.

Keywords Sarcoptes scabiei
Psoroptes ovis
Dermatophagoides pteronyssinus
genome size
DOI http://dx.doi.org/doi:10.1186/1756-3305-5-3   (check subscription with CDU E-Gateway service for CDU Staff and Students  check subscription with CDU E-Gateway in new window)
Additional Notes This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


© copyright

Every reasonable effort has been made to ensure that permission has been obtained for items included in CDU eSpace. If you believe that your rights have been infringed by this repository, please contact digitisation@cdu.edu.au.

 
Versions
Version Filter Type
Access Statistics: 34 Abstract Views, 36 File Downloads  -  Detailed Statistics
Created: Fri, 17 Jan 2014, 01:28:46 CST