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Comparison of TaqMan PCR assays for detection of the melioidosis agent Burkholderia pseudomallei in clinical specimens

Kaestli, Mirjam E., Richardson, Leisha J., Colman, Rebecca, Tuanyok, Apichai, Price, Erin P., Bowers, Jolene, Mayo, Mark J., Kelley, Erin, Seymour, Meagan, Sarovich, Derek, Pearson, Talima, Engelthaler, David M., Wagner, David M., Keim, Paul S., Schupp, James and Currie, Bart J. (2012). Comparison of TaqMan PCR assays for detection of the melioidosis agent Burkholderia pseudomallei in clinical specimens. Journal of Clinical Microbiology,50(6):2059-2062.

Document type: Journal Article
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NHMRC Grant No. 605820
Title Comparison of TaqMan PCR assays for detection of the melioidosis agent Burkholderia pseudomallei in clinical specimens
Author Kaestli, Mirjam E.
Richardson, Leisha J.
Colman, Rebecca
Tuanyok, Apichai
Price, Erin P.
Bowers, Jolene
Mayo, Mark J.
Kelley, Erin
Seymour, Meagan
Sarovich, Derek
Pearson, Talima
Engelthaler, David M.
Wagner, David M.
Keim, Paul S.
Schupp, James
Currie, Bart J.
Journal Name Journal of Clinical Microbiology
Publication Date 2012
Volume Number 50
Issue Number 6
ISSN 0095-1137   (check CDU catalogue  open catalogue search in new window)
Scopus ID 2-s2.0-84862075828
Start Page 2059
End Page 2062
Total Pages 4
Place of Publication Washington, United States
Publisher American Society for Microbiology
HERDC Category C1 - Journal Article (DIISR)
Abstract Melioidosis is an emerging infectious disease caused by the soil bacterium Burkholderia pseudomallei. In diagnostic and forensic settings, molecular detection assays need not only high sensitivity with low limits of detection but also high specificity. In a direct comparison of published and newly developed TaqMan PCR assays, we found the TTS1-orf2 assay to be superior in detecting B. pseudomallei directly from clinical specimens. The YLF/BTFC multiplex assay (targeting the Yersinia-like fimbrial/Burkholderia thailandensis-like flagellum and chemotaxis region) also showed high diagnostic sensitivity and provides additional information on possible geographic origin.
DOI http://dx.doi.org/10.1128/JCM.06737-11   (check subscription with CDU E-Gateway service for CDU Staff and Students  check subscription with CDU E-Gateway in new window)


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