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The genetic and molecular basis of O-antigenic diversity in Burkholderia pseudomallei lipopolysaccharride.

Tuanyok, Apichai, Stone, Joshua K., Mayo, Mark J., Kaestli, Mirjam E., Gruendike, Jeffrey, Georgia, Shalamar, Warrington, Stephanie, Mullins, Travis, Allendar, Christopher J., Wagner, David M., Chantratitia, Narisara, Peacock, Sharon J., Currie, Bart J. and Keim, Paul (2012). The genetic and molecular basis of O-antigenic diversity in Burkholderia pseudomallei lipopolysaccharride.. PLoS Neglected Tropical Diseases,6(1):e1453.

Document type: Journal Article
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Title The genetic and molecular basis of O-antigenic diversity in Burkholderia pseudomallei lipopolysaccharride.
Author Tuanyok, Apichai
Stone, Joshua K.
Mayo, Mark J.
Kaestli, Mirjam E.
Gruendike, Jeffrey
Georgia, Shalamar
Warrington, Stephanie
Mullins, Travis
Allendar, Christopher J.
Wagner, David M.
Chantratitia, Narisara
Peacock, Sharon J.
Currie, Bart J.
Keim, Paul
Journal Name PLoS Neglected Tropical Diseases
Publication Date 2012
Volume Number 6
Issue Number 1
ISSN 1935-2727   (check CDU catalogue open catalogue search in new window)
Scopus ID 2-s2.0-84856569017
Start Page e1453
Total Pages 9
Place of Publication United states
Publisher Public Library of Science
HERDC Category C1 - Journal Article (DIISR)
Abstract Lipopolysaccharide (LPS) is one of the most important virulence and antigenic components of Burkholderia pseudomallei, the causative agent of melioidosis. LPS diversity in B. pseudomallei has been described as typical, atypical or rough, based upon banding patterns on SDS-PAGE. Here, we studied the genetic and molecular basis of these phenotypic differences. Bioinformatics was used to determine the diversity of genes known or predicted to be involved in biosynthesis of the O-antigenic moiety of LPS in B. pseudomallei and its near-relative species. Multiplex-PCR assays were developed to target diversity of the O-antigen biosynthesis gene patterns or LPS genotypes in B. pseudomallei populations. We found that the typical LPS genotype (LPS genotype A) was highly prevalent in strains from Thailand and other countries in Southeast Asia, whereas the atypical LPS genotype (LPS genotype B) was most often detected in Australian strains (~13.8%). In addition, we report a novel LPS ladder pattern, a derivative of the atypical LPS phenotype, associated with an uncommon O-antigen biosynthesis gene cluster that is found in only a small B. pseudomallei sub-population. This new LPS group was designated as genotype B2. We also report natural mutations in the O-antigen biosynthesis genes that potentially cause the rough LPS phenotype. We postulate that the diversity of LPS may correlate with differential immunopathogenicity and virulence among B. pseudomallei strains.
DOI http://dx.doi.org/10.1371/journal.pntd.0001453   (check subscription with CDU E-Gateway service for CDU Staff and Students  check subscription with CDU E-Gateway in new window)


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