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Quantitative PCR confirms culture as the gold standard for detection of lower airway infection by nontypeable Haemophilus influenzae in Australian Indigenous children with bronchiectasis

Hare, Kim M., Marsh, Robyn L., Binks, Michael J., Grimwood, Keith, Pizzutto, Susan J., Leach, Amanda J., Chang, Anne B. and Smith-Vaughan, Heidi C. (2012). Quantitative PCR confirms culture as the gold standard for detection of lower airway infection by nontypeable Haemophilus influenzae in Australian Indigenous children with bronchiectasis. Journal of Microbiological Methods,92(3):270-272.

Document type: Journal Article
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IRMA ID MENbsnellxPUB41
Title Quantitative PCR confirms culture as the gold standard for detection of lower airway infection by nontypeable Haemophilus influenzae in Australian Indigenous children with bronchiectasis
Author Hare, Kim M.
Marsh, Robyn L.
Binks, Michael J.
Grimwood, Keith
Pizzutto, Susan J.
Leach, Amanda J.
Chang, Anne B.
Smith-Vaughan, Heidi C.
Journal Name Journal of Microbiological Methods
Publication Date 2012
Volume Number 92
Issue Number 3
ISSN 0167-7012   (check CDU catalogue open catalogue search in new window)
Scopus ID 2-s2.0-84873742757
Start Page 270
End Page 272
Total Pages 3
Place of Publication Netherlands
Publisher Elsevier BV
HERDC Category C1 - Journal Article (DIISR)
Abstract Correlation was observed between quantitative PCR and semi-quantitative culture for definition of Haemophilus influenzae infection in bronchoalveolar lavage specimens from 81 children with bronchiectasis. However, qPCR data correlated less well with airway neutrophilia, and supports continued use of culture as the gold standard for defining H. influenzae lower airway infection.
Keywords Airway cellularity
Bronchiectasis
Estimates of infection
Haemophilus haemolyticus
Lower airway infection
Nontypeable
Haemophilus influenzae
DOI http://dx.doi.org/10.1016/j.mimet.2012.12.013   (check subscription with CDU E-Gateway service for CDU Staff and Students  check subscription with CDU E-Gateway in new window)


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