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Degradation of [Dha7]MC-LR by a Microcystin Degrading Bacterium Isolated from Lake Rotoiti, New Zealand

Somdee, Theerasak, Thunders, Michelle, Ruck, John, Lys, Isabelle Y., Allison, Margaret and Page, Rachel (2013). Degradation of [Dha7]MC-LR by a Microcystin Degrading Bacterium Isolated from Lake Rotoiti, New Zealand. ISRN Microbiology,2013:596429-1-596429-8.

Document type: Journal Article
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IRMA ID 82794376xPUB272
Title Degradation of [Dha7]MC-LR by a Microcystin Degrading Bacterium Isolated from Lake Rotoiti, New Zealand
Author Somdee, Theerasak
Thunders, Michelle
Ruck, John
Lys, Isabelle Y.
Allison, Margaret
Page, Rachel
Journal Name ISRN Microbiology
Publication Date 2013
Volume Number 2013
ISSN 2090-7478   (check CDU catalogue open catalogue search in new window)
Start Page 596429-1
End Page 596429-8
Total Pages 8
Place of Publication United States of America
Publisher Hindawi Publishing Corporation
HERDC Category C1 - Journal Article (DIISR)
Abstract For the first time a microcystin-degrading bacterium (NV-3 isolate) has been isolated and characterized from a NZ lake. Cyanobacterial blooms in New Zealand (NZ) waters contain microcystin (MC) hepatotoxins at concentrations which are a risk to animal and human health. Degradation of MCs by naturally occurring bacteria is an attractive bioremediation option for removing MCs from drinking and recreational water sources. The NV-3 isolate was identified by 16S rRNA sequence analysis and found to have 100% nucleotide sequence homology with the Sphingomonas MC-degrading bacterial strain MD-1 from Japan. The NV-3 isolate (concentration of 1.0 × 108CFU/mL) at 30°C degraded a mixture of [Dha7]MC-LR and MC-LR (concentration 25μg/mL) at a maximum rate of 8.33μg/mL/day. The intermediate by-products of [Dha7]MC-LR degradation were detected and similar to MC-LR degradation by-products. The presence of three genes (mlrA, mlrB, and mlrC), that encode three enzymes involved in the degradation of MC-LR, were identified in the NV-3 isolate. This study confirmed that degradation of [Dha7]MC-LR by the Sphingomonas isolate NV-3 occurred by a similar mechanism previously described for MC-LR by Sphingomonas strain MJ-PV (ACM-3962). This has important implications for potential bioremediation of toxic blooms containing a variety of MCs in NZ waters.

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Additional Notes This is an Open Access article distributed under the terms of the Creative Commons Attribution License 3.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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