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Systematic Review and Consensus Guidelines for Environmental Sampling of Burkholderia pseudomallei

Limmathurotsakul, Direk, Dance, David A. B., Wuthiekanun, Vanaporn, Kaestli, Mirjam, Mayo, Mark J., Warner, Jeffrey M., Wagner, David M., Tuanyok, Apichai, Wertheim, Heiman, Cheng, Tan Yoke, Mukhopadhyay, Chiranjay, Puthucheary, Savithiri, Day, Nicholas P. J., Steinmetz, Ivo, Currie, Bart J. and Peacock, Sharon J. (2013). Systematic Review and Consensus Guidelines for Environmental Sampling of Burkholderia pseudomallei. PLoS Neglected Tropical Diseases,7(3):e2105-1-e2105-11.

Document type: Journal Article
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Title Systematic Review and Consensus Guidelines for Environmental Sampling of Burkholderia pseudomallei
Author Limmathurotsakul, Direk
Dance, David A. B.
Wuthiekanun, Vanaporn
Kaestli, Mirjam
Mayo, Mark J.
Warner, Jeffrey M.
Wagner, David M.
Tuanyok, Apichai
Wertheim, Heiman
Cheng, Tan Yoke
Mukhopadhyay, Chiranjay
Puthucheary, Savithiri
Day, Nicholas P. J.
Steinmetz, Ivo
Currie, Bart J.
Peacock, Sharon J.
Journal Name PLoS Neglected Tropical Diseases
Publication Date 2013
Volume Number 7
Issue Number 3
ISSN 1935-2735   (check CDU catalogue open catalogue search in new window)
Scopus ID 2-s2.0-84876012642
Start Page e2105-1
End Page e2105-11
Total Pages 11
Place of Publication United States of America
Publisher Public Library of Science
HERDC Category C1 - Journal Article (DIISR)
Abstract Background
Burkholderia pseudomallei, a Tier 1 Select Agent and the cause of melioidosis, is a Gram-negative bacillus present in the environment in many tropical countries. Defining the global pattern of B. pseudomallei distribution underpins efforts to prevent infection, and is dependent upon robust environmental sampling methodology. Our objective was to review the literature on the detection of environmental B. pseudomallei, update the risk map for melioidosis, and propose international consensus guidelines for soil sampling.

Methods/Principal Findings
An international working party (Detection of Environmental Burkholderia pseudomallei Working Party (DEBWorP)) was formed during the VIth World Melioidosis Congress in 2010. PubMed (January 1912 to December 2011) was searched using the following MeSH terms: pseudomallei or melioidosis. Bibliographies were hand-searched for secondary references. The reported geographical distribution of B. pseudomallei in the environment was mapped and categorized as definite, probable, or possible. The methodology used for detecting environmental B. pseudomallei was extracted and collated. We found that global coverage was patchy, with a lack of studies in many areas where melioidosis is suspected to occur. The sampling strategies and bacterial identification methods used were highly variable, and not all were robust. We developed consensus guidelines with the goals of reducing the probability of false-negative results, and the provision of affordable and ‘low-tech’ methodology that is applicable in both developed and developing countries.

The proposed consensus guidelines provide the basis for the development of an accurate and comprehensive global map of environmental B. pseudomallei.

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Additional Notes This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Description for Link Link to CC Attribution 4.0 License

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