Charles Darwin University

CDU eSpace
Institutional Repository

 
CDU Staff and Student only
 

Antibody Responses to Sarcoptes scabiei Apolipoprotein in a Porcine Model: Relevance to Immunodiagnosis of Recent Infection

Rampton, Melanie, Walton, Shelley F., Holt, Deborah C., Pasay, Cielo, Kelly, Andrew, Currie, Bart J., McCarthy, James S. and Mounsey, Kate E. (2013). Antibody Responses to Sarcoptes scabiei Apolipoprotein in a Porcine Model: Relevance to Immunodiagnosis of Recent Infection. PLoS One,8(6):e65354-1-e65354-10.

Document type: Journal Article
Attached Files (Some files may be inaccessible until you login with your CDU eSpace credentials)
Name Description MIMEType Size Downloads
Download this reading Holt_40620.pdf Published version application/pdf 1.00MB 101
Reading the attached file works best in Firefox, Chrome and IE 9 or later.

IRMA ID bsmithxPUB184
Title Antibody Responses to Sarcoptes scabiei Apolipoprotein in a Porcine Model: Relevance to Immunodiagnosis of Recent Infection
Author Rampton, Melanie
Walton, Shelley F.
Holt, Deborah C.
Pasay, Cielo
Kelly, Andrew
Currie, Bart J.
McCarthy, James S.
Mounsey, Kate E.
Journal Name PLoS One
Publication Date 2013
Volume Number 8
Issue Number 6
ISSN 1932-6203   (check CDU catalogue  open catalogue search in new window)
Scopus ID 2-s2.0-84878776881
Start Page e65354-1
End Page e65354-10
Total Pages 10
Place of Publication United States of America
Publisher Public Library of Science
HERDC Category C1 - Journal Article (DIISR)
Abstract No commercial immunodiagnostic tests for human scabies are currently available, and existing animal tests are not sufficiently sensitive. The recombinant Sarcoptes scabiei apolipoprotein antigen Sar s 14.3 is a promising immunodiagnostic, eliciting high levels of IgE and IgG in infected people. Limited data are available regarding the temporal development of antibodies to Sar s 14.3, an issue of relevance in terms of immunodiagnosis. We utilised a porcine model to prospectively compare specific antibody responses to a primary infestation by ELISA, to Sar s 14.3 and to S. scabiei whole mite antigen extract (WMA). Differences in the antibody profile between antigens were apparent, with Sar s 14.3 responses detected earlier, and declining significantly after peak infestation compared to WMA. Both antigens resulted in >90% diagnostic sensitivity from weeks 8–16 post infestation. These data provide important information on the temporal development of humoral immune responses in scabies and further supports the development of recombinant antigen based immunodiagnostic tests for recent scabies infestations.

DOI http://dx.doi.org/10.1371/journal.pone.0065354   (check subscription with CDU E-Gateway service for CDU Staff and Students  check subscription with CDU E-Gateway in new window)
Open access True
Additional Notes This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Description for Link Link to CC Attribution 4.0 License
URL https://creativecommons.org/licenses/by/4.0/au/legalcode


© copyright

Every reasonable effort has been made to ensure that permission has been obtained for items included in CDU eSpace. If you believe that your rights have been infringed by this repository, please contact digitisation@cdu.edu.au.

 
Versions
Version Filter Type
Access Statistics: 48 Abstract Views, 101 File Downloads  -  Detailed Statistics
Created: Thu, 07 Aug 2014, 17:17:11 CST