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Accurate and Rapid Identification of the Burkholderia pseudomallei Near-Neighbour, Burkholderia ubonensis, Using Real-Time PCR

Price, Erin P., Sarovich, Derek S., Webb, Jessica R., Ginther, Jennifer L., Mayo, Mark J., Cook, James M., Seymour, Meagan L., Kaestli, Mirjam E., Theobald, Vanessa, Hall, Carina M., Busch, Joseph D., Foster, Jeffrey T., Keim, Paul, Wagner, David M., Tuanyok, Apichai, Pearson, Talima and Currie, Bart J. (2013). Accurate and Rapid Identification of the Burkholderia pseudomallei Near-Neighbour, Burkholderia ubonensis, Using Real-Time PCR. PLoS One,8(8):e71647-1-e71647-6.

Document type: Journal Article
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Title Accurate and Rapid Identification of the Burkholderia pseudomallei Near-Neighbour, Burkholderia ubonensis, Using Real-Time PCR
Author Price, Erin P.
Sarovich, Derek S.
Webb, Jessica R.
Ginther, Jennifer L.
Mayo, Mark J.
Cook, James M.
Seymour, Meagan L.
Kaestli, Mirjam E.
Theobald, Vanessa
Hall, Carina M.
Busch, Joseph D.
Foster, Jeffrey T.
Keim, Paul
Wagner, David M.
Tuanyok, Apichai
Pearson, Talima
Currie, Bart J.
Journal Name PLoS One
Publication Date 2013
Volume Number 8
Issue Number 8
ISSN 1932-6203   (check CDU catalogue  open catalogue search in new window)
Scopus ID 2-s2.0-84881490139
Start Page e71647-1
End Page e71647-6
Total Pages 6
Place of Publication United States of America
Publisher Public Library of Science
HERDC Category C1 - Journal Article (DIISR)
Abstract Burkholderia ubonensis is an environmental bacterium belonging to the Burkholderia cepacia complex (Bcc), a group of genetically related organisms that are associated with opportunistic but generally nonfatal infections in healthy individuals. In contrast, the near-neighbour species Burkholderia pseudomallei causes melioidosis, a disease that can be fatal in up to 95% of cases if left untreated. B. ubonensis is frequently misidentified as B. pseudomallei from soil samples using selective culturing on Ashdown’s medium, reflecting both the shared environmental niche and morphological similarities of these species. Additionally, B. ubonensis shows potential as an important biocontrol agent in B. pseudomallei-endemic regions as certain strains possess antagonistic properties towards B. pseudomallei. Current methods for characterising B. ubonensis are laborious, time-consuming and costly, and as such this bacterium remains poorly studied. The aim of our study was to develop a rapid and inexpensive real-time PCR-based assay specific for B. ubonensis. We demonstrate that a novel B. ubonensis-specific assay, Bu550, accurately differentiates B. ubonensis from B. pseudomallei and other species that grow on selective Ashdown’s agar. We anticipate that Bu550 will catalyse research on B. ubonensis by enabling rapid identification of this organism from Ashdown’s-positive colonies that are not B. pseudomallei.

DOI http://dx.doi.org/10.1371/journal.pone.0071647   (check subscription with CDU E-Gateway service for CDU Staff and Students  check subscription with CDU E-Gateway in new window)
Additional Notes This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Description for Link Link to CC Attribution 4.0 License
URL https://creativecommons.org/licenses/by/4.0/au/legalcode


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