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Inhibition of Streptococcus pneumoniae adherence to human epithelial cells in vitro by the probiotic Lactobacillus rhamnosus GG

Wong, Sook-San, Toh, Zheng, Dunne, Eileen, Mulholland, E. Kim, Tang, Mimi, Robins-Browne, Roy, Licciardi, Paul V. and Satzke, Catherine (2013). Inhibition of Streptococcus pneumoniae adherence to human epithelial cells in vitro by the probiotic Lactobacillus rhamnosus GG. BMC Research Notes,6:135-1-135-7.

Document type: Journal Article
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Title Inhibition of Streptococcus pneumoniae adherence to human epithelial cells in vitro by the probiotic Lactobacillus rhamnosus GG
Author Wong, Sook-San
Toh, Zheng
Dunne, Eileen
Mulholland, E. Kim
Tang, Mimi
Robins-Browne, Roy
Licciardi, Paul V.
Satzke, Catherine
Journal Name BMC Research Notes
Publication Date 2013
Volume Number 6
ISSN 1756-0500   (check CDU catalogue  open catalogue search in new window)
Scopus ID 2-s2.0-84875823963
Start Page 135-1
End Page 135-7
Total Pages 7
Place of Publication United Kingdom
Publisher BioMed Central Ltd.
HERDC Category C1 - Journal Article (DIISR)
Abstract Background
Colonization of the nasopharynx by Streptococcus pneumoniae is considered a prerequisite for pneumococcal infections such as pneumonia and otitis media. Probiotic bacteria can influence disease outcomes through various mechanisms, including inhibition of pathogen colonization. Here, we examine the effect of the probiotic Lactobacillus rhamnosus GG (LGG) on S. pneumoniae colonization of human epithelial cells using an in vitro model. We investigated the effects of LGG administered before, at the same time as, or after the addition of S. pneumoniae on the adherence of four pneumococcal isolates.

Results
LGG significantly inhibited the adherence of all the pneumococcal isolates tested. The magnitude of inhibition varied with LGG dose, time of administration, and the pneumococcal isolate used. Inhibition was most effective when a higher dose of LGG was administered prior to establishment of pneumococcal colonization. Mechanistic studies showed that LGG binds to epithelial cells but does not affect pneumococcal growth or viability. Administration of LGG did not lead to any significant changes in host cytokine responses.

Conclusions
These findings demonstrate that LGG can inhibit pneumococcal colonization of human epithelial cells in vitro and suggest that probiotics could be used clinically to prevent the establishment of pneumococcal carriage.
Keywords Probiotic
LGG
Pneumococci
Colonization
in viro model
DOI http://dx.doi.org/10.1186/1756-0500-6-135   (check subscription with CDU E-Gateway service for CDU Staff and Students  check subscription with CDU E-Gateway in new window)
Additional Notes This is an Open Access article distributed under the terms of the Creative Commons Attribution License 3.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Description for Link Link to CC Attribution 3.0 License
URL https://creativecommons.org/licenses/by/3.0/au/legalcode


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