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Genetic variability in phytoplasmas associated with papaya yellow crinkle and papaya mosaic diseases in Queensland and the Northern Territory

De La Rue, Stephanie J., Schneider, Bernd L. and Gibb, Karen S. (1999). Genetic variability in phytoplasmas associated with papaya yellow crinkle and papaya mosaic diseases in Queensland and the Northern Territory. Australasian Plant Pathology,28(2):108-114.

Document type: Journal Article
Citation counts: Scopus Citation Count Cited 10 times in Scopus Article | Citations

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Title Genetic variability in phytoplasmas associated with papaya yellow crinkle and papaya mosaic diseases in Queensland and the Northern Territory
Author De La Rue, Stephanie J.
Schneider, Bernd L.
Gibb, Karen S.
Journal Name Australasian Plant Pathology
Publication Date 1999
Volume Number 28
Issue Number 2
ISSN 0815-3191   (check CDU catalogue open catalogue search in new window)
Scopus ID 2-s2.0-0033442683
Start Page 108
End Page 114
Total Pages 7
Place of Publication Netherlands
Publisher Springer Netherlands
Field of Research 060704 - Plant Pathology
Abstract Samples of papaya (Carzca papaya L.) showing symptoms of papaya yellow crinkle (PYC) or papaya mosaic (PM) diseases were collected from two study sites in Queensland (Rockhampton and Caboolture) and from Katherine in the Northern Territory. Phytoplasmas were detected in these samples using polymerase chain reaction (PCR) and fluorescence microscopy techniques. The genetic variability of the phytoplasmas was studied using Southern blot hybridisation, restriction fragment length polymorphism (RFLP), and sequence analysis. RFLP analysis of PYC samples collected from Caboolture indicated that only the tomato big bud (TBB) type phytoplasma was present, whereas both PYC and PM samples collected from Rockhampton exhibited two phytoplasma types, sweet potato little leaf variant (SPLL-V4) and TBB. PYC samples collected from Katherine showed even greater variability with cactus witches’ broom (CWB), which was first described from Indonesia, detected in addition to SPLL-V4 and TBB which are common throughout Australia. Although PYC and PM diseases have different symptoms, no genetically distinct phytoplasma could be found in exclusive association with either disease.
Keywords PCR
RFLP
DOI http://dx.doi.org/10.1071/AP99019   (check subscription with CDU E-Gateway service for CDU Staff and Students  check subscription with CDU E-Gateway in new window)
 
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Created: Fri, 29 Aug 2014, 19:55:14 CST by Anthony Hornby