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14C- and 3H-tyrosine incorporation into Ascidia ceratodes tunichrome in vivo

Robinson, William E., Kustin, Kenneth, Matulef, Kimberly, Parry, David L., He, Xiaoying and Ryan, Evelyn K. (1996). 14C- and 3H-tyrosine incorporation into Ascidia ceratodes tunichrome in vivo. Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology,115(4):475-481.

Document type: Journal Article
Citation counts: Scopus Citation Count Cited 4 times in Scopus Article | Citations

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Title 14C- and 3H-tyrosine incorporation into Ascidia ceratodes tunichrome in vivo
Author Robinson, William E.
Kustin, Kenneth
Matulef, Kimberly
Parry, David L.
He, Xiaoying
Ryan, Evelyn K.
Journal Name Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
Publication Date 1996
Volume Number 115
Issue Number 4
ISSN 0305-0491   (check CDU catalogue open catalogue search in new window)
Scopus ID 2-s2.0-0030448993
Start Page 475
End Page 481
Total Pages 7
Place of Publication United States of America
Publisher Elsevier Inc.
Field of Research 250000 Chemical Sciences
Abstract Radiolabeled tyrosine is incorporated into newly biosynthesized tunichrome of Ascidia ceratodes at a rate comparable to previous findings with phenylalanine. 3H-tyrosine incorporation indicates that formation of the catechol and pyrogallol moieties occurs via the NIH shift pathway. Radiolabel is found in many compartments other than solely tunichrome (tunic > whole body ⪢ blood plasma ⋙ tunichrome). The amount of label in the tunic, whole bodies and blood plasma declines over the 14 days following exposure to 3H-tyrosine, whereas the radiolabel in the tunichrome continues to increase. These results are consistent with two alternative hypotheses: tunichromes may be formed either by sequential hydroxylation of di- and tri-peptide precursors, or by selective cleavage of a larger DOPA- or TOPA-containing compound. In either event, hydroxylation may precede formation of tunichrome's carbon backbone.
Keywords Biosynthesis
Hemocyte
Natural product
NIH shift
Tunicate
Tunichrome
Tyrosine
Vanadium
DOI http://dx.doi.org/10.1016/S0305-0491(96)00190-3   (check subscription with CDU E-Gateway service for CDU Staff and Students  check subscription with CDU E-Gateway in new window)
 
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Created: Fri, 29 Aug 2014, 20:14:44 CST by Anthony Hornby