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A comparison of the phytoplasma associated with Australian grapevine yellows to other phytoplasmas in grapevine

Padovan, Anna C., Gibb, Karen S., Daire, X. and Boudon-Padieu, E. (1996). A comparison of the phytoplasma associated with Australian grapevine yellows to other phytoplasmas in grapevine. Vitis,35(4):189-194.

Document type: Journal Article
Citation counts: Scopus Citation Count Cited 40 times in Scopus Article | Citations
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Title A comparison of the phytoplasma associated with Australian grapevine yellows to other phytoplasmas in grapevine
Author Padovan, Anna C.
Gibb, Karen S.
Daire, X.
Boudon-Padieu, E.
Journal Name Vitis
Publication Date 1996
Volume Number 35
Issue Number 4
ISSN 0042-7500   (check CDU catalogue  open catalogue search in new window)
Scopus ID 2-s2.0-0030498534
Start Page 189
End Page 194
Total Pages 6
Place of Publication Germany
Publisher Julius Kuehn-Institut, Bundesforschungsinstitut fuer Kulturpflanzen * Institut fuer Rebenzuechtung Geilweilerhof
Field of Research 1108 - Medical Microbiology
Abstract The phytoplasma associated with Australian grapevine yellows (AGY) was compared to other phytoplasma diseases of grapevine using the polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) analysis .
Comparison of eight different Australian isolates suggests that only one type of phytoplasma is associated with this disease. Based on RFLP analysis of the 16S rRNA gene, it was shown that AGY is different from the tomato big bud and sweet potato little leaf phytoplasma strains which are widespread in Australia and that it represents the only other phytoplasma strain recorded in Australia to date . Restriction profiles of grapevine phytoplasmas using Mse I suggest that AGY is unique but most closely resembles those phytoplasmas associated with grapevine diseases in the stolbur group. Sequence analysis of the 16S rRNA gene and adjacent spacer region supports this association . The uniqueness of AGY was confirmed by PCR assays using non-ribosomal primers ; the primer pair STOL1lfl/r2 specific for stolbur phytoplasmas did not result in amplification products in grapevines affected with AGY; the primer pair fML01/rML01 which amplifies a region of the tufgene from phytoplasmas in the aster yellows cluster, amplified AGY DNA confirming its association within this phylogenetic group . RFLP analysis of the tufPCR product again highlighted a distinction between AGY and other stolbur phytoplasmas occurring in grapevine . The only other phytoplasma in Australia which is in the stolbur group is associated with dieback in papaya, and it has the same RFLP profile of the tufPCR product as AGY.
Keywords Australian grapevine yellows
PCR
Phytoplasma
RFLP
Description for Link Link to published version
URL http://www.vitis-vea.de/admin/volltext/e037981.pdf


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